基于自支撑多孔硅微腔的DNA光学检测  

DNA Optical Sensing Based on Self-Supporting Porous Silicon Microcavities

作　　者：马仲伟 谢洁荣 吕长武[1] MA Zhongwei;XIE Jierong;LYU Changwu(School of Physics and Technology,Xinjiang University,Urumqi Xinjiang 830017,China)

机构地区：[1]新疆大学物理科学与技术学院,新疆乌鲁木齐830017

出　　处：《新疆大学学报（自然科学版）（中英文）》2023年第2期196-201,共6页Journal of Xinjiang University(Natural Science Edition in Chinese and English)

基　　金：新疆维吾尔自治区自然科学基金“基于多孔硅光栅光学生物传感器的研究”(2018D01C055)。

摘　　要：用电化学蚀刻在单晶硅表面制备了多孔硅微腔(PSM),并通过大电流脉冲分离制成了一种自支撑多孔硅微腔结构.将该结构用于8个碱基对的DNA片段检测,根据折射率变化引起透射角度谱的移动量检测与探针DNA分子发生偶联反应的目标DNA分子的不同浓度.目标DNA分子在0.1~1.0μmol/L的浓度区间,透射角度谱红移量随着目标DNA分子浓度的增加而增大,并且有较好的线性关系,检测限达0.037μmol/L.自支撑多孔硅微腔检测生物分子时表现出灵敏度高、响应时间快、免标记、成本低等优点,可应用于便携式光学传感器.Porous silicon microcavities(PSM)were prepared on the surface of single crystal silicon by electro-chemical etching,and a self-supporting porous silicon microcavity structure was fabricated by high current pulse separation.The structure was used for the detection of eight base pairs of DNA fragments,and the different concentrations of the target DNA molecules coupled to the probe DNA molecules were detected according to the shift of the transmission angle spectrum caused by the change of refractive index.The red shift of the transmission angle spectrum increases with the increases of the target DNA molecules concentrations in the concentration range of 0.1»1.0μmol/L,and has good linearity with a detection limit of 0.037μmol/L.Self-supporting porous silicon microcavities exhibit high sensitivity,fast response time,label-free and low costs when detecting biomolecules,which can be applied to portable optical sensors.

关 键 词：自支撑多孔硅微腔 透射角度谱 DNA传感 

分 类 号：O433.4[机械工程—光学工程]