捏脊疗法对自闭症模型大鼠焦虑样行为及NF-κB通路的影响  

Effect of Chiropractic Therapy on Anxiety-like Behaviorand NF-κB Pathway in Rats with Autism

作　　者：龚梦华 吴倩雯 李一纯 唐佳瑶 林丽莉 GONG Meng-hua;WU Qian-wen;LI Yi-chun;TANG Jia-yao;LIN Li-li(Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Fujian Academy of Traditional Chinese Medicine,Fuzhou 350003,China)

机构地区：[1]福建中医药大学,福建福州350122 [2]福建省中医药科学院,福建福州350003

出　　处：《云南中医中药杂志》2023年第4期81-85,共5页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：国家自然科学基金项目(81704194)。

摘　　要：目的基于NF-κB通路探究捏脊疗法对自闭症模型大鼠焦虑样行为及海马神经炎症的影响。方法将12只Wistar孕鼠随机分为正常组和造模组,每组6只。于妊娠12.5 d,2组孕鼠均按体质量0.24 mL/100 g,腹腔注射给药,造模组腹腔注射250 mg/mL丙戊酸钠溶液(VPA),正常组腹腔注射等体积生理盐水;所产子代经睁眼试验、方向趋向性实验筛选后确定为自闭症模型大鼠。造模成功后将自闭症模型大鼠随机分为模型组和捏脊组,每组12只;正常组随机选取12只作为正常对照组。捏脊组出生后第23 d开始干预,每天1次,21遍/次,连续干预30 d。末次干预后采用旷场实验检测大鼠焦虑水平,Western blot检测大鼠海马组织中NF-κB p65、TNFR 1和TNFR 2蛋白表达,Real-time PCR检测大鼠海马组织中TNF-α、IKKαmRNA表达水平。结果与正常组仔鼠比较,造模组仔鼠睁眼时间更晚,方向趋向性机能降低(P<0.01);与正常对照组比较,模型组Rd值下降(P<0.01),NF-κB p65、TNFR1蛋白和TNF-αmRNA表达升高(P<0.01);与模型组比较,捏脊组Rd值明显上升(P均<0.01),NF-κB p65、TNFR 1蛋白和TNF-αmRNA表达降低(P<0.01);3组TNFR 2蛋白和IKKαmRNA表达水平无差异(P>0.05)。结论捏脊疗法可有效改善自闭症大鼠焦虑样行为,其作用可能是通过抑制NF-κB通路的过度激活而实现的。Objective:To study the effect of chiropractic therapy on anxiety-like behavior and hippocampal neuroinflammation in rats with autism based on NF-κB pathway.Methods:12 Wistar pregnant mice were randomly divided into normal group and modeling group,6 mice each group.At 12.5 days of gestation,the pregnant mice in the two groups were injected intraperitoneally according to their body weight of 0.24mL/100g.The modeling group was injected intraperitoneally with 250 mg/mL sodium valproate solution(VPA),and the normal group was injected intraperitoneally with normal saline.The offspring were selected by eye opening test and orientation test as autistic model rats.The model rats were randomly divided into model group and chiropractic group,12 rats each group.12 rats in the normal group were randomly selected as the normal control group.In the chiropractic group,intervention began on the 23rd day after birth,once a day,21 times/time,continuous intervention for 30 days.After the last intervention,the anxiety level of the rats was detected by open field test,the protein expressions of NF-κB p65,TNFR 1 and TNFR 2 in the hippocampus were detected by Western Blot,and the mRNA expressions of TNF-αand IKKαin the hippocampus were detected by Real-time PCR.Results:Compared with that of the normal group,the eye opening time of the rats in the model group was later and the direction-oriented function was decreased(P<0.01).Compared with that in the normal control group,Rd value in the model group was decreased(P<0.01),and mRNA expressions of NF-κB p65,TNFR1 protein and TNF-αwere increased(P<0.01).Compared with that in the model group,Rd value in the chiropractic group was significantly increased(P<0.01),and mRNA expressions of NF-κB p65,TNFR 1 protein and TNF-αwere decreased(P<0.01).There were no differences in the expression levels of TNFR 2 protein and IKKαmRNA among the three groups(P>0.05).Conclusion:Chiropractic therapy can effectively improve anxiety-like behavior in autistic rats,which may be achieved by inhibiting the

关 键 词：捏脊疗法 自闭症 NF-ΚB通路 焦虑样行为 

分 类 号：R244.1[医药卫生—针灸推拿学]