植物源miR156a-5p对五指山断奶仔猪肠道菌群和上皮屏障的影响  

Barrier of Wuzhishan Weaned Piglets

作　　者：王奕 孙瑞萍[2] 彭永佳 姜涵晨 马飞 张瑾[1] 黎梦 WANG Yi;SUN Ruiping;PENG Yongjia;JIANG Hanchen;MA Fei;ZHANG Jin;LI Meng(College of Biological Chemical Sciences and Engineering,Jiaxing University,Jiaxing 314000,China;Institute of Animal Science and Veterinary Medicine,Hainan Academy of Agricultural Science,Haikou 571100,China)

机构地区：[1]嘉兴学院生物与化学工程学院,嘉兴314000 [2]海南省农业科学院畜牧兽医研究所,海口571100

出　　处：《动物营养学报》2023年第4期2184-2195,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金资助项目(32002244);浙江省自然科学基金资助项目(LY22C180005);三亚崖州湾科技城管理局科研项目(SKJC-2020-02-007);国家重点研发项目(2021YFF0702804);国家自然科学基金资助项目(32202714);国家级大学生创新创业训练计划资助项目(202110354032)。

摘　　要：本试验旨在探究植物源miR156a-5p对五指山断奶仔猪肠道菌群和上皮屏障的影响。选取初始体重为(5.82±0.13)kg的35日五指山龄断奶仔猪12头,随机分为2组,每组6个重复,每个重复1头。对照组饲喂无意义单链核酸,试验组饲喂合成的植物源miR156a-5p(每天600 pmol/kg)。试验期7 d。结果表明:与对照组相比,1)试验组的终末体重和平均日增重均无显著差异(P>0.05),肺脏指数显著增加(P<0.05);2)试验组的血清白蛋白和高密度脂蛋白胆固醇含量显著或极显著提高(P<0.05);3)试验组十二指肠Chao1指数和Observed_otus指数显著提高(P<0.05),空肠Simpson指数显著降低(P<0.05),盲肠Shanon指数和Simpson指数显著或极显著提高(P<0.05或P<0.01),结肠Simpson指数显著提高(P<0.05);4)试验组十二指肠拟杆菌目(Bacteroidales)、拟杆菌纲(Bacteroidia)和拟杆菌属(Bacteroides)的富集显著增加(P<0.05),十二指肠纤毛菌属(Leptotrichia)、颤螺旋菌属(Oscillibacter)、UCG-010、螺杆菌属(Helicobacter)、脱硫弧菌属(Desulfovibrio)、Clostridia_vadinBB60_group、副萨特氏菌属(Parasutterella)和理研菌属(Rikenella)等的富集显著增加(P<0.05);5)试验组十二指肠pH和隐窝深度极显著提高(P<0.01);6)试验组十二指肠载脂蛋白A1(APOA1)和尾型同源盒转录因子2(CDX2)mRNA相对表达量极显著上调(P<0.01),十二指肠黏糖蛋白2(MUC2)、肿瘤坏死因子-α(TNF-α)和β防御素1(DEFB1)mRNA相对表达量显著上调(P<0.05)。综上所述,植物源miR156a-5p可调节断奶五指山仔猪十二指肠、空肠、盲肠和结肠菌群α多样性,增加十二指肠pH及拟杆菌纲、目和属水平的富集,改善十二指肠上皮分化、化学屏障和黏膜免疫屏障功能。This study aimed to investigate the effects of plant miR156a-5p on intestinal microbiota and epithelial barrier of Wuzhishan weaned piglets.Twelve 35-day-old Wuzhishan weaned piglets with initial body weight of(5.82±0.13)kg were randomly divided into 2 groups with 6 replicates per group and one piglet per replicate.Piglets in the control group were fed nonsense single-stranded nucleic acids,and others in experimental group were fed synthetic plant miR156a-5p(600 pmol/kg per day).The experiment lasted for 7 days.The results showed that compared with the control group,1)the final body weight and average daily gain of experimental group were no significant difference(P>0.05),and the lung index was significantly increased(P<0.05);2)the serum albumin and high-density lipoprotein cholesterol contents of experimental group were significantly increased(P<0.05 or P<0.01);3)the duodenal Chao1 index and Observed_otus index of experimental group were significantly increased(P<0.05),the jejunal Simpson index was significantly decreased(P<0.05),the cecal Shanon index and Simpson index were significantly increased(P<0.05 or P<0.01),and the colonic Simpson index was significantly increased(P<0.05);4)the enrichment of Bacteroidales,Bacteroidia,and Bacteroides in duodenum of experimental group were significantly increased(P<0.05),and the enrichment of Leptotrichia,Oscillibacter,UCG-010,Helicobacter,Desulfovibrio,Clostridia_vadinBB60_group,Parasutterella and Rikenella in duodenum were significantly increased(P<0.05);5)the duodenal crypt depth and pH of experimental group were significantly increased(P<0.01);6)the mRNA relative expression levels of apolipoprotein A1(APOA1)and tail-type homology cassette transcription factor 2(CDX2)in duodenum of experimental group were significantly increased(P<0.01),and the mRNA relative expression levels of mucin 2(MUC2),tumor necrosis factor-α(TNF-α)andβdefensin 1(DEFB1)in duodenum were significantly increased(P<0.05).In conclusion,plant miR156a-5p can regulate theαvariety of microbiota in d

关 键 词：植物源miRNA 断奶仔猪 肠道菌群 肠道屏障 

分 类 号：S828[农业科学—畜牧学]