产肠毒素大肠杆菌K88对BALB/c小鼠肝脏炎症反应、程序性坏死和焦亡信号通路关键基因表达的影响  

Effects of Enterotoxigenic Escherichia coli K88 on Expression of Key Genes in Liver Inflammatory Response,Necroptosis and Pyroptosis Signaling Pathways in Liver of BALB/c Mice

作　　者：连欣 王东方[1] 杨阳 刘玉兰[1] 胡进 LIAN Xin;WANG Dongfang;YANG Yang;LIU Yulan;HU Jin(Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Wuhan 430023,China)

机构地区：[1]武汉轻工大学,动物营养与饲料科学湖北省重点实验室,武汉430023

出　　处：《动物营养学报》2023年第4期2661-2667,共7页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：武汉市科技局知识创新专项基础研究项目(2022020801010391);武汉轻工大学引进(培养)人才科研启动资金资助(2021RZ087)。

摘　　要：本试验旨在研究产肠毒素大肠杆菌(ETEC)K88对BALB/c小鼠肝脏炎症反应、程序性坏死和焦亡信号通路关键基因表达的影响。试验选取25只9周龄、体重(17±1)g的雌性BALB/c小鼠,随机分为5个组,每组5只,每只小鼠腹腔注射1×10^(7) CFU/mL ETEC K88菌液0.2 mL;分别于注射ETEC K880、12、24、36和48 h时屠宰小鼠,取肝脏组织测定炎性因子、程序性坏死和焦亡信号通路关键基因mRNA的表达。结果表明:1)与0 h相比,小鼠肝脏中炎性因子肿瘤坏死因子-α(TNF-α)的mRNA相对表达量在24、36和48 h时显著升高(P<0.05),白细胞介素-1β(IL-1β)的mRNA相对表达量在12和36 h时显著升高(P<0.05),白细胞介素-6(IL-6)的mRNA相对表达量在36和48 h时显著升高(P<0.05);2)与0 h相比,小鼠肝脏中受体相互作用蛋白激酶1(RIPK1)的mRNA相对表达量在12 h时显著升高(P<0.05),受体相互作用蛋白激酶3(RIPK3)的mRNA相对表达量在36 h时显著升高(P<0.05),混合谱系激酶结构域样假激酶(MLKL)的mRNA相对表达量在12、36和48 h时显著升高(P<0.05);3)与0 h相比,小鼠肝脏中核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)的mRNA相对表达量在36 h时显著升高(P<0.05),凋亡相关斑点样蛋白(ASC)的mRNA相对表达量在12、36和48 h时显著升高(P<0.05),半胱氨酸天冬氨酸蛋白酶-1(Caspase-1)的mRNA相对表达量在24和36 h时显著升高(P<0.05),消皮素D(GSDMD)的mRNA相对表达量在12、24和36 h时显著升高(P<0.05),白细胞介素-18(IL-18)的mRNA相对表达量在24 h时显著升高(P<0.05)。综上所述,ETEC K88感染引起小鼠肝脏炎症反应,激活了小鼠肝脏程序性坏死和焦亡信号通路。This experiment was conducted to investigate the effects of enterotoxigenic Escherichia coli(ETEC)K88 on the expression of key genes in inflammatory response,necroptosis and pyroptosis signaling pathways in liver of BALB/c mice.Twenty-five nine-week-old female BALB/c mice weighing(17±1)g were randomly divided into 5 groups with 5 mice in each group.Each mouse was intraperitoneally injected with 0.2 mL 1×10^(7) CFU/mL ETEC K88 bacterial solution.Mice were slaughtered at 0,12,24,36 and 48 h after ETEC K88 injection,and liver tissues were taken to determine the mRNA expressions of key genes in inflammatory factors,necroptosis and pyroptosis signaling pathways.The results showed as follows:1)compared with 0 h,the inflammatory factor tumor necrosis factor-α(TNF-α)mRNA relative expression level in liver of mice was significantly increased at 24,36 and 48 h(P<0.05),the interleukin-1β(IL-1β)mRNA relative expression level was significantly increased at 12 and 36 h(P<0.05),and the interleukin-6(IL-6)mRNA relative expression level was significantly increased at 36 and 48 h(P<0.05).2)Compared with 0 h,the receptor-interacting protein kinase 1(RIPK1)mRNA relative expression level in liver of mice was significantly increased at 12 h(P<0.05),the receptor-interacting protein kinase 3(RIPK3)mRNA relative expression level was significantly increased at 36 h(P<0.05),and the mixed lineage kinase domain like pseudokinase(MLKL)mRNA relative expression level was significantly increased at 12,36 and 48 h(P<0.05).3)Compared with 0 h,the nucleotide binding oligomeric domain-like receptor protein 3(NLRP3)mRNA relative expression level of in liver of mice was significantly increased at 36 h(P<0.05),the apoptosis-associated speck-like protein(ASC)mRNA relative expression level was significantly increased at 12,36 and 48 h(P<0.05),the cysteine-aspartic protease-1(Caspase-1)mRNA relative expression level was significantly increased at 24 and 36 h(P<0.05),the gasdermin D(GSDMD)mRNA relative expression level was significantly increased at

关 键 词：产肠毒素大肠杆菌 肝脏 炎症 程序性坏死 焦亡 

分 类 号：S811[农业科学—畜牧学]