Visible light assisted enzyme-photocatalytic cascade degradation of organophosphorus pesticides  

作　　者：Ying Zhang Xue Cao Yufeng Yang Sumin Guan Xiaotian Wang Heyu Li Xiaobing Zheng Liya Zhou Yanjun Jiang Jing Gao 

机构地区：[1]School of Chemical Engineering and Technology,Hebei University of Technology,Tianjin,300130,China [2]Hebei Xinlan Environmental Protection Engineering Co.Ltd.,Baoding,071000,China [3]Tianjin UBasio Biotechnology Group Co.,Ltd.,Tianjin,300457,China [4]National Local Joint Engineering Laboratory for Energy Conservation of Chemical Process Integration and Resources Utilization,Hebei University of Technology,Tianjin,300130,China

出　　处：《Green Chemical Engineering》2023年第1期30-38,共9页绿色化学工程（英文）

基　　金：supported by the National Natural Science Foundation of China(Nos.21901058,21908040,and 21878068);Tianjin Enterprise Science and Technology Commissioner,China(21YDTPJC00810);Science Technology Research Project of Higher Education of Hebei Province,China(QN2021045);Hebei Province Postgraduate Innovation Funding Project,China(CXZZSS2021027);National College Student’s Science and Technology Innovation Project,China(202010080038)。

摘　　要：The worldwide application of organophosphorus pesticides(OPs)has promoted agricultural development,but their gradual accumulation in soil and water can seriously affect the central nervous system of humans and other mammals.Organophosphorus hydrolase(OPH)is an effective enzyme that can catalyze the degradation of the residual OPs.However,the degradation products such as p-nitrophenol(p-NP)is still toxic.Thus,it is of great significance to develop a multi-functional support that can be simultaneously used for the immobilization of OPH and the further degradation of p-NP.Herein,a visible light assisted enzyme-photocatalytic integrated catalyst was constructed by immobilizing OPH on hollow structured Au-TiO_(2)(named OPH@H-Au-TiO_(2))for the degradation of OPs.The obtained OPH@H-Au-TiO_(2)can degrade methyl parathion to p-NP by OPH and then degrade p-NP to hydroquinone with low toxicity by using H-Au-TiO_(2)under visible light.OPH molecules were immobilized on HAu-TiO_(2)through adsorption method to prepare OPH@H-Au-TiO_(2).After 2.5 h of reaction,methyl parathion is completely degraded,and about 82.64%of the generated p-NP is further degraded into hydroquinone.After reused for 4 times,the OPH@H-Au-TiO_(2)retains more than 80%of the initial degradation activity.This research presents a new insight in designing and constructing multi-functional biocatalyst,which greatly expands the application scenarios and industrial value of enzyme catalysis.

关 键 词：Organophosphorus pesticides Multi-functional biocatalyst Enzyme-photocatalytic cascade catalyst Immobilized enzyme Organophosphorus hydrolase 

分 类 号：TQ426.97[化学工程] X592[环境科学与工程—环境工程]