OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast development  

作　　者：CHEN Chang-zhao WANG Ya-liang HE Meng-xing LI Zhi-wen SHEN Lan LI Qing REN Deyong HU Jiang ZHU Li ZHANG Guang-heng GAO Zhen-yu ZENG Da-li GUO Long-biao QIAN Qian ZHANG Qiang 

机构地区：[1]State Key Lab of Rice Biology,China National Rice Research Institute,Hangzhou 310006,P.R.China [2]College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua 321004,P.R.China [3]College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 310006,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第4期972-980,共9页农业科学学报（英文版）

基　　金：funded by the Central Public-Interest Scientific Institution Basal Research Fund,China(CPSIBRF-CNRRI-202111 and CPSIBRF-CNRRI-202110);the Agricultural Science and Technology Innovation Program,Chinese Academy of Agricultural Sciences(ASTIP);the Project of State Key Laboratory of Rice Biology,China(2020ZZKT10205);the Key Research and Development Project of China Rice Research Institute(CNRRI-2020-01)。

摘　　要：Photosynthesis occurs mainly in chloroplasts,whose development is regulated by proteins encoded by nuclear genes.Among them,pentapeptide repeat(PPR)proteins participate in organelle RNA editing.Although there are more than 450 members of the PPR protein family in rice,only a few affect RNA editing in rice chloroplasts.Gene editing technology has created new rice germplasm and mutants,which could be used for rice breeding and gene function study.This study evaluated the functions of OsPPR9 in chloroplast RNA editing in rice.The osppr9 mutants were obtained by CRISPR/Cas9,which showed yellowing leaves and a lethal phenotype,with suppressed expression of genes associated with chloroplast development and accumulation of photosynthetic-related proteins.In addition,loss of OsPPR9 protein function reduces the editing efficiency of rps8-C182,rpoC2-C4106,rps14-C80,and ndhB-C611 RNA editing sites,which affects chloroplast growth and development in rice.Our data showed that OsPPR9 is highly expressed in rice leaves and encodes a DYW-PPR protein localized in chloroplasts.Besides,the OsPPR9 protein was shown to interact with OsMORF2 and OsMORF9.Together,our findings provide insights into the role of the PPR protein in regulating chloroplast development in rice.

关 键 词：rice(Oryza sativa L.) PPR protein chloroplast development RNA editing 

分 类 号：S511[农业科学—作物学]