Characterization of the microbial community response to replant diseases in peach orchards  

作　　者：LI Wei-hua CHEN Peng WANG Yu-zhu LIU Qi-zhi 

机构地区：[1]Laboratory of Entomology and Nematology,College of Plant Protection,China Agricultural University,Beijing 100193,P.R.China [2]State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,P.R.China [3]Key Laboratory of Natural Enemies Insects of Ministry of Agriculture and Rural Affairs,Shandong Provincial Engineering Technology Research Center on Biocontrol of Crop Diseases and Insect Pest,Institute of Plant Protection,Shandong Academy of Agricultural Sciences,Jinan 250100,P.R.China [4]Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第4期1082-1092,共11页农业科学学报（英文版）

基　　金：supported by the National Science and Technology Support Program of China(2014BAD20B01 and 2014BAD16B07);the National Key R&D Program of China(2019YFE0120400);the Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences,China(CXGC2021B13 and CXGC2022D06)。

摘　　要：This study attempted to monitor the development of microbial communities and reveal the correlation between the soil microbial community and soil nutrient factors over different years following the replanting of peach trees.The replanted soil(RS)and nonreplanted soil(NRS)were collected from peach orchards with different growth years(1,3,5,7,9,11,and 13 years)in the same region.The soil bacterial and fungal community diversities were analyzed by high-throughput sequencing technology.Redundancy analysis(RDA)was used to show the correlation between the soil microbial community and environmental variables.The alpha diversities of the bacterial and fungal communities indicated that RS contained a higher abundance of bacterial and fungal operational taxonomic units(OTUs)than NRS.NMDS and ANOSIM analyses showed that the soil bacterial and fungal communities were significantly(P<0.01)affected by planting years,and that the main changes occurred in the first and ninth planting years.The presence of the bacterial orders Sphingobacteriales,Burkholderiales and Actinomycetales changed significantly after replanting.Some bacteria associated with bioremediation,such as Burkholderiales and Intrasporangiaceae,and some harmful pathogens,such as Penicillium and Ophiostomatales,significantly increased after replanting(LDA score>3.0).In addition,the soil nutrient contents were lower in RS than in NRS in the early stage(1–5 years),and the RDA showed that bacterial and fungal phyla are closely associated with environmental variables,including the potential of hydrogen(pH),ammonium nitrogen(AN),available phosphorus(AP)and available potassium(AK).These results lead to a deeper understanding of the microbial responses to replanting in peach orchards.

关 键 词：replant disease complex syndrome microbial community high-throughput sequencing environmental variables 

分 类 号：S436.619[农业科学—农业昆虫与害虫防治]