Green manuring facilitates bacterial community dispersal across different compartments of subsequent tobacco  

作　　者：LIANG Hai FU Li-bo CHEN Hua ZHOU Guo-peng GAO Song-juan CAO Wei-dong 

机构地区：[1]College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,P.R.China [2]Institute of Agricultural Environment and Resources,Yunnan Academy of Agricultural Sciences,Kunming 650205,P.R.China [3]Key Laboratory of Plant Nutrition and Fertilizer,Ministry of Agriculture and Rural Affairs/Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第4期1199-1215,共17页农业科学学报（英文版）

基　　金：supported financially by the National Key Research and Development Program of China(2021YFD1700200);the China Agriculture Research System of MOF and MARA(CARS-22).

摘　　要：Green manure–crop rotation is a sustainable approach to protect crops against diseases and improve yield.However,the mechanism by which green manuring manipulates the crop-associated microbial community remains to be elucidated.In this study,we explored the horizontal processes of bacterial communities in different compartments of the soil–root interface(bulk soil,rhizosphere soil,rhizoplane and endosphere)of tobacco by performing a field experiment including four rotation practices,namely,tobacco rotated with smooth vetch,ryegrass,radish,and winter fallow(without green manure).Results showed that the co-occurrence networks constructed by adjacent compartments of the soil–root interface with green manuring had more edges than without green manuring,indicating dramatic microbial interactions.Green manuring increased the dispersal-niche continuum index between bulk soil and other compartments,indicating that it facilitated the horizontal dispersal of microbes.For the different green manuring practices,the neutral community model explained 24.6–27.6%of detection frequency for bacteria,and at least one compartment under each practice had a normalized stochasticity ratio higher than the 50%boundary point,suggesting that the deterministic and stochastic processes jointly shaped the tobacco microbiome.In conclusion,green manuring generally facilitates bacterial community dispersal across different compartments and enhances potential interactions among adjacent compartments.This study provides empirical evidence for understanding the microbiome assembly under green manure–crop rotation.

关 键 词：green manure TOBACCO rotation practice niche compartment community assembly 

分 类 号：S142[农业科学—肥料学] S572[农业科学—农业基础科学]