Alginate/gelatin/boron-doped hydroxyapatite-coated Ti implants:in vitro and in vivo evaluation of osseointegration  

作　　者：Ahmet E.Pazarçeviren Zafer Evis Tayfun Dikmen Korhan Altunbas Mustafa V.Yaprakçı Dilek Keskin Aysen Tezcaner 

机构地区：[1]Department of Engineering Sciences,Middle East Technical University,Ankara,Turkey [2]Department of Histology and Embryology,Afyon Kocatepe University,Afyon,Turkey [3]Department of Surgery,Afyon Kocatepe University,Afyon,Turkey [4]Center of Excellence in Biomaterials and Tissue Engineering,Ankara,Turkey

出　　处：《Bio-Design and Manufacturing》2023年第3期217-242,共26页生物设计与制造（英文）

基　　金：Acknowledgements Authors would like to thank Center of Excellence in Biomaterials and Tissue Engineering(BIOMATEN)for the support provided.Authors also acknowledge financial support provided by National Boron Institute(BOREN,Grant No:2018-31-07-25-001).

摘　　要：In this study,boron-doped hydroxyapatite(BHT)-loaded alginate/gelatin-based(A/G)hydrogel coating on Ti was fabricated to support bone integration through triggering osteoinduction,vascularization and immunomodulation.Initially,highly reproducible,cheap and time-effective BHT was produced,which significantly promoted higher osteogenic and angiogenic maturation,while a mild innate immune response was observed.The immense potential of BHT was evidenced by the production of a gap-filling A/G/BHT interphase on Ti implants to mimic the osseous extracellular matrix to achieve functional bridging and exert control over the course of innate immune response.We initially aminosilanized the implant surface using 3-aminopropyl triethoxysilane,and then coated it with 0.25%w/v alginate with 20 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-hydroxysuccinimide to allowthe A/G/BHT pre-gel to disperse evenly and covalently attach on the surface.The pre-gel was added with 0.2 M NaCl to homogeneously blend BHT in the structure without inducing ionic crosslinking.Then,the coated implants were freeze-dried and stored.The coated layer demonstrated high cohesive and adhesive strength,and 8-month-long shelf-life at room temperature and normal humidity.The A/G/BHT was able to coat an irregularly shaped Ti implant.Osteoblasts and endothelial cells thrived on the A/G/BHT,and it demonstrated greatly improved osteogenic and angiogenic capacity.Moreover,A/G/BHT maintained macrophage viability and generated an acute increase in immune response that could be resolved rapidly.Finally,A/G/BHT was shown to induce the robust integration of implant in a rabbit femur osteochondral model within 2months.Therefore,we concluded that A/G/BHT coatings could serve as amultifunctional reservoir,promoting the strong and rapid osseointegration of metallic implants.

关 键 词：Boron Alginate/gelatin Implant coating Titanium Osteochondral model 

分 类 号：TG174.4[金属学及工艺—金属表面处理] R783.6[金属学及工艺—金属学]