阿司匹林对SD大鼠的致畸作用  

Teratogenic effect of aspirin on SD rats

作　　者：武昕[1] 黎耀俊[1] 潘晓慧 郑佳琳[1] 严家荣[1] WU Xin;LI Yao-jun;PAN Xiao-hui;ZHENG Jia-lin;YAN Jia-rong(Guangdong Medical Laboratory Animal Center,Foshan 528248,Guangdong,China)

机构地区：[1]广东省医学实验动物中心,广东佛山528248

出　　处：《广东医学》2023年第3期307-311,共5页Guangdong Medical Journal

摘　　要：目的总结汇总我实验室2015年以来使用阿司匹林作为致畸试验阳性对照药的结果,供开展此类实验的同行们参考。方法将受孕的雌鼠随机分为阴性对照组、阿司匹林组,每组50只。受孕第6~15天灌胃给予阿司匹林280 mg/kg体重,1次/d,共10 d。受孕第20天处死孕鼠,剖腹检查受孕情况和胚胎发育情况,记录子宫连胎重、着床数、黄体数、活胎数、吸收胎数、死胎数。对胎仔进行外观检查,逐一记录胎仔体重、性别、身长、尾长。检查胎仔外观后,每窝的1/2胎仔用95%的乙醇固定,茜素红染色后进行骨骼检查,另1/2胎仔用Bouins液固定后进行内脏检查。结果与阴性对照组相比,阿司匹林组受孕大鼠GD12、GD15、GD20体重减轻,流产率增加,子宫连胎重减轻、活胎率减少、窝均活胎数减少、吸收胎率增加,胎盘重量减轻、胎仔体重减轻、胎仔身长和尾长短小,外观畸形、骨骼畸形、内脏畸形的发生率增加,差异均有统计学意义(P<0.05)。结论在本实验条件下,280 mg/kg体重的阿司匹林对SD大鼠有明显的致畸性。Objective To summarize the results of using aspirin as a positive control in teratogenic test since 2015 in our laboratory.Methods The pregnant female rats were randomly divided into negative control group and aspirin group(n=50).On the 6th to 15th day of conception,aspirin 280 mg/kg was given by gavage,once a day,for 10 consecutive days.Pregnant rats were sacrificed on the 20th day of conception,and the pregnancy and embryonic development were checked by caesarean section.The uterine contiguous weight,number of implantation,number of corpus luteum,number of live fetuses,number of absorbed fetuses,and number of stillbirths were recorded.The fetuses were visually inspected,and the weight,sex,body length,and tail length of the fetuses were recorded.After checking the appearance of the fetuses,1/2 of the fetuses were fixed with 95%ethanol,skeletal examination was performed after alizarin red staining.The internal organs were examined using the other 1/2 fetuses with Bouins solution fixation.Results Compared with the negative control group,the GD12,GD15 and GD20 weights of the pregnant rats in the aspirin group were decreased,the abortion rate was increased,the uterine contiguous weight was decreased,the live fetus was rate was decreased,the average number of live litters was decreased,the absorbed fetus rate was increased,and the weight of the placenta was increased.The incidence of appearance deformity,skeletal deformity,and visceral deformity increased,all of these were statistically significant(P<0.05).Conclusion Under the experimental conditions,aspirin of 280 mg/kg showed obvious teratogenicity to SD rats.

关 键 词：阿司匹林 SD大鼠 致畸 阳性对照 

分 类 号：R96[医药卫生—药理学] R-33[医药卫生—药学]