The micronuclear histone H3 clipping in the unicellular eukaryote Tetrahymena thermophila  

作　　者：Fan Wei Bo Pan Jinghan Diao Yuanyuan Wang Yalan Sheng Shan Gao 

机构地区：[1]Institute of Evolution and Marine Biodiversity,Ocean University of China,Qingdao,266003,China [2]Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao,266237,China

出　　处：《Marine Life Science & Technology》2022年第4期584-594,共11页海洋生命科学与技术（英文）

基　　金：supported by the National Natural Science Foundation of China(32125006,32070437);China National Postdoctoral Program for Innovative Talents(BX2021277);China Postdoctoral Science Foundation(2021M690144);Natural Science Foundation of Shandong Province of China(ZR2021QC046);Postdoctoral Applied Research Project of Qingdao.

摘　　要：Clipping of the histone H3 N-terminal tail has been implicated in multiple fundamental biological processes for a growing list of eukaryotes.H3 clipping,serving as an irreversible process to permanently remove some post-translational modifications(PTMs),may lead to noticeable changes in chromatin dynamics or gene expression.The eukaryotic model organism Tetrahymena thermophila is among the first few eukaryotes that exhibits H3 clipping activity,wherein the first six amino acids of H3 are cleaved off during vegetative growth.Clipping only occurs in the transcriptionally silent micronucleus of the binucleated T.thermophila,thus offering a unique opportunity to reveal the role of H3 clipping in epigenetic regulation.However,the physiological functions of the truncated H3 and its protease(s)for clipping remain elusive.Here,we review the major findings of H3 clipping in T.thermophila and highlight its association with histone modifications and cell cycle regulation.We also summarize the functions and mechanisms of H3 clipping in other eukaryotes,focusing on the high diversity in terms of protease families and cleavage sites.Finally,we predict several protease candidates in T.thermophila and provide insights for future studies.

关 键 词：Histone H3 tail-H3 clipping protease Histone post-translational modifications(PTMs)-Cell development 

分 类 号：Q75[生物学—分子生物学]