超声、紫外及加温对质粒介导的黏菌素耐药基因mcr-1的影响  

The Influence of Ultrasound,Ultraviolet Radiation and Heating on Plasmid-Mediated Colistin Resistance Gene mcr-1

作　　者：王立琦 吕世明 王忠 WANG Liqi;LV Shiming;WANG Zhong(Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education,College of Animal Science,Guizhou University,Guiyang,Guizhou 550025 China)

机构地区：[1]贵州大学动物科学学院高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳550025

出　　处：《家畜生态学报》2023年第3期9-15,共7页Journal of Domestic Animal Ecology

基　　金：国家自然科学基金资助项目(32160853,32260829,31760750);贵州省自然科学基金项目(黔科合基础[2022]一般113)。

摘　　要：为探讨超声、紫外线照射及加温等方法对质粒介导的黏菌素耐药基因mcr-1的影响,采用四因素三水平正交试验设计,以mcr-1阳性猪场废水样品为研究对象,采用实时荧光定量聚合酶链反应(qRT-PCR)测定mcr-1基因绝对丰度,以处理前后mcr-1基因绝对丰度变化率为指标,考察不同因素及水平对mcr-1基因含量的影响。结果表明,超声对mcr-1丰度有显著影响,其次是温度、加温时间,而紫外线照射对mcr-1的影响甚微。当依次将样品进行超声30 min、紫外线照射15 min、温度40℃作用30 min时,会使mcr-1含量比对照组高5倍多。而当超声时间为15 min、同时提高温度至50℃作用30 min和60℃作用5 min时,可使mcr-1的含量分别降低20.5%和30.6%。可见,不同因素及水平的组合可对mcr-1基因的含量造成相反的影响。因此,在实际中应筛选最佳的条件以尽可能地降低mcr-1基因的含量,减少mcr-1基因的进一步污染和扩散。To investigate the effects of ultrasound,ultraviolet radiation and heating on plasmid-mediated colistin resistance gene mcr-1,a four-factor and three-level orthogonal experiment was conducted.The absolute abundance of mcr-1 gene in pig wastewater samples before and after treatment was determined by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).The results showed that ultrasonography had a significant effect on the abundance of mcr-1,followed by temperature and heating time,while ultraviolet irradiation had little effect on it.When the samples were treated with ultrasound for 30 min,ultraviolet radiation for 15min at 40℃ for 30 min,the abundance of mcr-1 was more than 5 times higher than that of control group.When the ultrasonic time was set at 15 min and the temperature was increased to 50℃ for 30min or to 60℃ for 5min,the abundance of mcr-1 was reduced by 20.5% and 30.6%,respectively.This indicate that the combination of different factors and levels showed opposite effects on abundance of mcr-1.Therefore,in practice,conditions should be optimized to reduce mcr-1 gene as much as possible,so as to reduce its further contamination and diffusion.

关 键 词：mcr-1 紫外 超声 加温 QRT-PCR 

分 类 号：S852.6[农业科学—基础兽医学]