延髓头端腹外侧区在电针预处理减轻心肌缺血再灌注损伤中的作用  

Effect of rostral ventrolateral medulla in electroacupuncture pretreatment for alleviating myocardial ischemia-reperfusion

作　　者：余情[1,2] 吴立斌 张帆 魏小桐[1] 乙春梅 蔡荣林 胡玲 Qing YU;Li-bin WU;Fan ZHANG;Xiao-tong WEI;Chun-mei YI;Rong-lin CAI;Ling HU(College of Acupuncture and Moxibustion,Anhui University of Chinese Medicine,Hefei 230012,China;Acupuncture and Meridian Research Institute,Anhui Academy of Chinese Medicine,Hefei 230038,China;Key Laboratory of Xin'an Medicine,Ministry of Education,Anhui University of Chinese Medicine,Hefei 230038,China)

机构地区：[1]安徽中医药大学针灸推拿学院,安徽合肥230012 [2]安徽省中医药科学院针灸经络研究所,安徽合肥230038 [3]新安医学教育部重点实验室,安徽合肥230038

出　　处：《World Journal of Acupuncture-Moxibustion》2023年第2期142-149,共8页世界针灸杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China:8197375,82074536,82104999;Cultivation of Outstanding and Top Talents in Universities of Anhui Province:gxgwfx2019025;Nature Science Research Project of Anhui province:2108085Y30,2108085QH36。

摘　　要：ObjectiveTo observe the effects of pretreatment with electroacupuncture (EA) on neuron activity in the rostral ventrolateral medulla (RVLM) of rats with myocardial ischemia-reperfusion injury (MIRI) and explore the central regulatory mechanism of EA in attenuating MIRI.MethodsOf 72 SD rats, 12 were randomly allocated into the group of EA pretreatment + RVLM nucleus damage (EA + RVLM lesion group). The other 60 rats were randomized (20 rats each) into a sham-operation group, a model group, and an EA pretreatment group (EA group). Except for the rats in the sham-operation group, the models of MIRI were prepared by ligating the left anterior descending coronary artery in the model, EA, and EA + RVLM lesion groups. The rats of the EA group were intervened with EA at “Shénmén (神门HT7)” and “Tōnglĭ (通里HT5)”, 1 mA in current intensity and 2 Hz in frequency, for 20 min each time per day. Before modeling, the intervention was given for seven consecutive days. In the EA + RVLM lesion group, 3 weeks after microinjection with the neuronal apoptotic virus at bilateral RVLM, the same EA intervention as the EA group was provided. Afterward, the MIRI models were prepared. In the model group, no EA intervention was given. Using Powerlab electrophysiolograph, ST segment displacement value and arrhythmia score were recorded and analyzed before modeling, 30 min after ligation, and 120 min after reperfusion in each group. The concentration of cardiac troponin (cTnl) was detected with an ELISA assay kit. Using immunofluorescence staining, the expression level of c-fos protein of RVLM was detected in the sham-operation, model, and EA groups separately. Plexon multichannel acquisition processor was adopted to record the neuronal firing and field potential of RVLM in the sham-operation, model, and EA groups.ResultsST segment displacement value, arrhythmia score, and cTnl concentration 30 min after ligation and 120 min after reperfusion were all elevated in the model group compared to the sham-operation group (all P < 0

关 键 词：ELECTROACUPUNCTURE Myocardial ischemia-reperfusion injury Rostral ventrolateral medulla Neural mechanism 

分 类 号：R245.97[医药卫生—针灸推拿学]