妊娠期糖尿病孕妇胎盘组织中PPARγmRNA和蛋白相对表达量的检测意义  

Detective significance of relative expression of PPARγmRNA and protein in placental tissues of pregnant women with gestational diabetes mellitus

作　　者：王文兴[1] 刘晶影 Wang Wenxing;Liu Jingying(Department of Obstetrics and Gynecology,Yantai Yaitaishan Hospital,Yantai 264001,China)

机构地区：[1]烟台市烟台山医院妇产科,烟台264001

出　　处：《中华内分泌外科杂志》2023年第2期244-248,共5页Chinese Journal of Endocrine Surgery

摘　　要：目的分析妊娠期糖尿病(gestational diabeies mellitus,GDM)孕妇脂肪组织及胎盘组织中过氧化物酶体增殖物激活受体(PPARγ)mRNA和蛋白相对表达量。方法选取2019年1月至2020年11月烟台市烟台山医院妇产科住院待产的GDM孕妇52例(观察组)与同时期门诊体检并分娩的正常孕妇50例(对照组),采集血清标本后检测胰岛细胞功能。两组孕妇分娩后采集其胎盘组织标本,测定胎盘组织与脂肪组织的PPARγmRNA及PPARγ蛋白含量,并分析GDM孕妇胎盘、脂肪组织PPARγmRNA及PPARγ蛋白与胰岛细胞功能的相关性。结果观察组HOMA-IR水平高于对照组[(3.45±1.06)%vs(1.40±0.43)%]、HOMA-β水平[(126.59±23.59)%vs(153.12±27.34)%]低于对照组(P<0.05)。观察组胎盘组织PPARγmRNA[(1.65±0.21)vs(0.93±0.16)]与PPARγ蛋白含量[(1.89±0.51)vs(1.02±0.23)]高于对照组(P<0.05),脂肪组织中PPARγmRNA[(0.49±0.12)vs(1.15±0.26)]及PPARγ蛋白含量[(0.43±0.11)%vs(0.96±0.22)%]低于对照组(P<0.05)。脂肪组织PPARγmRNA及PPARγ蛋白与HOMA-IR(r=-0.45,-0.33)呈负相关性,与HOMA-β(r=0.47,0.43)呈正相关性(P<0.05);胎盘组织PPARγmRNA及PPARγ蛋白与HOMA-IR(r=0.40,0.37)呈正相关性,与HOMA-β(r=-0.44,-0.35)呈负相关性(P<0.05)。结论PPARγmRNA与PPARγ蛋白水平在GDM患者脂肪组织中呈低表达,在胎盘组织中反馈性高表达,且PPARγ表达与HOMA-IR、HOMA-β均有明显相关性,可为GDM的临床治疗提供新靶点和方向。Objective To analyze the clinical value of relative expression of peroxisome proliferators-activated receptors mRNA(PPAR mRNA)and protein in placental tissues of pregnant women with gestational diabetes mellitus(GDM).Methods 52 pregnant women(the study group)with GDM who were admitted to our hospital from Jan.2019 to Nov.2020 and 50 normal pregnant women(the control group)who underwent physical examination and gave birth during the same period were selected.Serum samples were collected to detect the islet cell function.Placental tissue samples of the two groups of pregnant women were collected after delivery to measure the content of PPARγmRNA and protein in placental tissues and adipose tissues.The correlations between PPARγin placental,adipose tissues of GDM pregnant women with islet cell function were analyzed.Results The HOMA-IR level of the study group was higher than that of the control group[(3.45±1.06)%vs(1.40±0.43)%],and the HOMA-βlevel[(126.59±23.59)%vs(153.12±27.34)%]was lower than that of the control group(P<0.05).The PPARγmRNA[(1.65±0.21)vs(0.93±0.16)]and PPARγprotein content[(1.89±0.51)vs(1.02±0.23)]of placenta tissue in the study group were higher than those in the control group(P<0.05),the PPARγmRNA[(0.49±0.12)vs(1.15±0.26)]and PPARγprotein content[(0.43±0.11)%vs(0.96±0.22)%]in adipose tissue were lower than those of the control group(P<0.05).Adipose tissue PPARγmRNA and PPARγprotein were negatively correlated with HOMA-IR(r=-0.45,-0.33),and positively correlated with HOMA-β(r=0.47,0.43)(P<0.05);placental tissue PPARγmRNA and PPARγprotein were positively correlated with HOMA-IR(r=0.40,0.37),and negatively correlated with HOMA-β(r=-0.44,-0.35)(P<0.05).Conclusion The levels of PPARγmRNA and PPARγprotein are low expressed in adipose tissue of GDM patients,and highly expressed in placental tissues,and PPARγexpression is significantly correlated with HOMA-IR and HOMA-β,which can provide new clinical treatment for GDM Target and direction.

关 键 词：糖尿病 妊娠期糖尿病 胎盘组织 过氧化物酶体增殖物激活受体 胰岛素抵抗 

分 类 号：R714.256[医药卫生—妇产科学]