接种Forl对番茄幼苗生长、根系保护性酶及防御相关基因表达的影响  

Effects of Forl Inoculation on Tomato Seedling Growth,Protective Enzymes Activity and Relative Expression of Defense-related Genes

作　　者：王宇 李芬 王辉[1] 朱文莹 李文丽[1] 王富[1] WANG Yu;LI Fen;WANG Hui;ZHU Wenying;LI Wenli;WANG Fu(College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109)

机构地区：[1]青岛农业大学园艺学院,山东青岛266109

出　　处：《北方园艺》2023年第7期40-45,共6页Northern Horticulture

基　　金：山东省农业良种工程资助项目(2020LZGC005);山东省现代农业产业技术体系资助项目(SDAIT-05);青岛市科技惠民示范引导专项资助项目(21-1-4-ny-25-nsh);山东省重点研发计划(农业良种工程)资助项目(2021LZGC018)。

摘　　要：以番茄品种‘Moneymaker’幼苗为试材,采用人工接种番茄颈腐根腐病病原菌的方法,研究了接种该病原菌后番茄幼苗形态、生理及相关基因的相对表达量,病原菌对番茄幼苗生长、根系保护性酶活性及防御相关基因表达的影响,以期为该病防治提供参考依据。结果表明:与对照相比,接种12 d后,番茄幼苗主要生长指标显著低于对照,病情指数高达62.3%。随着接种天数增加,接种和对照处理幼苗根系活力均呈上升趋势,但接种处理增幅较为缓慢,且接种后6 d和12 d均显著低于对照;而超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性均显著高于对照。另外,随着接种天数增加,接种处理幼苗根系中乙烯信号标记基因ETR4与Pti4相对表达量均显著升高;水杨酸合成关键基因ICS的相对表达量显著增加,而PAL的相对表达变化幅度较小;防御信号基因PR1a的响应较为强烈,相对表达量最高升至200倍左右;而蛋白酶抑制剂基因PI-1的相对表达量变化较小。Seedlings of tomato cultivar‘Moneymaker’were used as test materials.Fusarium oxysporum f.sp.radicis-lycopersici(Forl)was inoculated with artificial inoculation method,root protective enzyme activity and defense-related gene expression,morphology,physiology and related genes expression of seedlings were studied after inoculation,the effects of Forl inoculation on seedling growth were studied,in order to provide reference for prevent the disease.The results showed that after 12 days of inoculation,the main growth indexes of tomato seedlings were significantly lower,and the disease index was up to 62.3%.With the increase of inoculation days,the root activity of seedlings in both inoculation and control treatments increased,but the increase of inoculation treatment was slow,and were significantly lower than control in 6 days and 12 days.SOD and CAT activities were significantly higher than those of the control.Furthermore,the relative expression levels of ethylene signal marker genes ETR4 and Pti4 increased significantly with the increase of inoculation days.The relative expression key gene ICS for salicylic acid synthesis,increased significantly,while the relative expression of PAL changed slightly.The response of PR1a defense signal gene was strong,and its relative expression was up to 200 times.The relative expression of protease inhibitor gene PI-1 had no change.

关 键 词：番茄 番茄颈腐根腐病病原菌 幼苗生长 保护性酶 防御基因 

分 类 号：S641.2[农业科学—蔬菜学]