不同脱毒处理怀地黄对干旱胁迫的响应特性  

Response Characteristics of Rehmannia glutinosa to Drought Stress Under Different Virus-free Treatments

作　　者：李萌 腊贵晓[3] 张宝[1,2] 苏秀红 董诚明[1,2] LI Meng;LA Guixiao;ZHANG Bao;SU Xiuhong;DONG Chengming(College of Pharmacy,Henan University of Traditional Chinese Medicine,Zhengzhou,Henan 450046;Henan Synergistic Innovation Center of Respiratory Disease Diagnosis and New Drug Development,Zhengzhou,Henan 450046;Institute of Economic Crops,Henan Provincial Academy of Agricultural Sciences,Zhengzhou,Henan 450002)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]呼吸疾病诊疗与新药研发河南省协同创新中心,河南郑州450046 [3]河南省农业科学院经济作物研究所,河南郑州450002

出　　处：《北方园艺》2023年第7期111-118,共8页Northern Horticulture

基　　金：河南省重点研发与推广专项资助项目(222102310625,222102310455);国家现代农业产业技术体系资助项目(CARS-21);河南省中药材产业技术体系资助项目(HARS-22-11-G2)。

摘　　要：以怀地黄的病毒苗、茎尖脱毒苗、“电”疗法脱毒苗为试材,采用不同浓度PEG 6000(0%、5%、10%、15%)模拟干旱胁迫的方法,研究了干旱胁迫对3种材料形态特征、叶绿素、叶片相对含水量、渗透调节物质及抗氧化酶活性的影响,以期为脱毒地黄在大田栽培上的应用推广提供参考依据。结果表明:PEG 6000胁迫下,3种材料生长明显受到抑制,生根天数延长,株高、生根数、根长、鲜质量均降低,但以“电”疗法获取的脱毒苗生长情况最好;随干旱胁迫程度增加,“电”疗法获取的脱毒苗叶绿素含量先下降后上升,茎尖培养的脱毒苗略微下降,而病毒苗则表现为下降、上升再下降的趋势;叶片相对含水量逐渐降低;而可溶性糖、可溶性蛋白质含量、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性随胁迫程度加深则呈现出持续上升的趋势,以“电”疗法脱毒苗含量最高。综上可知,“电”疗法脱毒苗抗旱能力较强,可为地黄抗旱机制的研究及田间栽培提供示范参考作用。Taking virus seedlings,shoot tip virus-free seedlings and‘electric’therapy virus-free seedlings as experimental materials,the effects of drought stress on morphological characteristics,chlorophyll,relative water content of leaves,osmoregulatory substances and antioxidant enzyme activities of the three materials were studied by simulating drought stress with different concentrations of PEG 6000(0%,5%,10%and 15%),in order to provide reference for the application and promotion of detoxified groundnut in field cultivation.The results showed that the growth of the three materials was significantly inhibited under PEG 6000 stress.With the extension of rooting days,plant height,rooting number,root length and fresh weight decreased,but the growth of virus-free seedlings obtained by‘electric’therapy was the best.With the increase of drought stress,the chlorophyll content of virus-free seedlings obtained by‘electric’therapy first decreased and then increased.The virus-free seedlings in shoot tip culture decreased slightly,while the virus-free seedlings showed a downward,upward and then downward trend.The relative water content of leaves decreased gradually.The contents of soluble sugar,soluble protein,and activities of CAT,SOD and POD showed a continuous upward trend with the deepening of stress,and the content of virus-free seedlings treated with‘electric’therapy was the highest.Therefore,the‘electric’treatment of virus-free seedlings had strong drought resistance,which could provide a demonstration reference for the study of drought resistance mechanism and field cultivation of Rehmannia glutinosa.

关 键 词：地黄 脱毒 PEG 6000 生长情况 生理特性 

分 类 号：S853.75[农业科学—临床兽医学]