基于5-羟色胺及其受体研究安眠巴布贴对对氯苯丙氨酸致失眠大鼠的影响  

Study of effects of Anmian Babu Plaster in rats with insomnia induced by p-chlorophenylalanine based on 5-hydroxytryptamine and its receptor

作　　者：赵娜[1] 谢晨[2] 李金金 于心同[1] 杨文佳[1] 陈云飞[1] ZHAO Na;XIE Chen;LI Jinjin;YU Xintong;YANG Wenjia;CHEN Yunfei(Yueyang Integrative Medicine Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai200437,China;Shanghai Institute of Acupuncture and Meridian,Shanghai200030,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437 [2]上海市针灸经络研究所,上海200030

出　　处：《中国医药导报》2023年第12期4-8,共5页China Medical Herald

基　　金：国家自然科学基金资助项目(81503631);上海市自然科学基金项目(21ZR1464300)。

摘　　要：目的基于5-羟色胺(5-HT)及其受体研究安眠巴布贴对对氯苯丙氨酸致失眠大鼠的影响。方法将48只体重(200±10)g的SPF级SD大鼠安装脑电监测电极后按随机数字表法分为对照组、模型组、干预组、安慰组,每组12只。模型组、干预组、安慰组按300 mg/(kg·d)腹腔注射对氯苯丙氨酸2 d制作失眠模型,对照组注射等量生理盐水。造模成功后,对照组及模型组常规饲养,干预组神阙穴敷贴安眠巴布贴,安慰组神阙穴敷贴安慰贴,连续干预7 d。观察四组睡眠-觉醒累积量,采用酶联免疫吸附试验、免疫组织化学染色检测四组中缝背核、下丘脑、海马中5-HT、5-羟吲哚乙酸(5-HIAA)及5-HT1A受体(5-HT1AR)、5-HT2AR表达。采用RT-PCR检测5-HT1AR mRNA、5-H2AR mRNA水平。结果模型组12 h白天、24 h全天觉醒量高于对照组,快速眼动(REM)、非快速眼动(NREM)睡眠量低于对照组,差异有统计学意义(P<0.05)。干预组12 h白天、24 h全天觉醒量低于模型组,REM、NREM睡眠量高于模型组,差异有统计学意义(P<0.05)。模型组中缝背核、下丘脑、海马组织5-HT、5-HIAA、5-HT1AR mRNA表达水平均低于对照组,5-HT2AR mRNA表达水平高于对照组,差异有统计学意义(P<0.05)。干预组中缝背核、下丘脑、海马组织5-HT、5-HIAA、5-HT1AR mRNA表达水平均高于模型组,5-HT2AR mRNA表达水平低于模型组,差异有统计学意义(P<0.05)。对照组和干预组中缝背核、下丘脑、海马组织5-HT1AR阳性细胞膜染色深数目多;模型组细胞膜染色浅且阳性细胞数目少。对照组和干预组中缝背核、下丘脑、海马组织5-HT2AR阳性细胞膜染色浅且数目少;模型组阳性细胞膜染色深且数目多。安慰组各指标与模型组比较,差异无统计学意义(P>0.05)。结论安眠巴布贴可能通过调节中枢5-HT及5-HT1AR、5-HT2AR表达来发挥对PCPA致失眠大鼠的治疗作用。Objective To study the effects of Anmian Babu Plaster in rats with insomnia induced by p-chlorophenylalanine based on 5-hydroxytryptamine(5-HT)and its receptor.Methods A total of 48 SPF SD rats with the body weight of(200±10)g were installed with electroencephalography monitoring electrodes and divided into control group,model group,intervention group,and comfort group according to random number table method,with 12 rats in each group.Model group,intervention group,and comfort group were intraperitoneally injected with 300 mg/(kg·d)p-chlorophenylalanine for two days to make insomnia model,and control group was injected with the same amount of normal saline.After successful modeling,the control group and model group were routinely fed,shénquēin the intervention group were pasted with Anmian Babu Plaster,and shénquēin the comfort group were pasted with comfort plaster,the intervention was lasted for seven days.The sleep-wake accumulations of the four groups were observed.The levels of 5-HT and 5-hydroxyindoleacetic acid(5-HIAA)and the expressions of 5-HT1A receptor(5-HT1AR)and 5-HT2AR in the dorsal raphe nucleus,hypothalamus,and hippocampus of the four groups were detected by enzyme-linked immunosorbent assay and immunohistochemical staining.The expression levels of 5-HT1ARmRNA and 5-H2ARmRNA were measured by RT-PCR.Results The 12 h daytime and 24 h all-day awakening volume of model group were higher than those of control group, while the rapid eye movement (REM) and non-rapid eye movement (NREM) sleep volume of model group were lower than those of control group, and the differences were statistically significant (P<0.05). The 12 h daytime and 24 h all-day awakening volume in the intervention group were lower than those in the model group, while the REM and NREM sleep volume were higher than those in the model group, and the differences were statistically significant (P<0.05). The levels of 5-HT, 5-HIAA, and the expression levels of 5-HT1AR mRNA in dorsal raphe nucleus, hypothalamus, and hippocampus in model

关 键 词：安眠巴布贴 失眠 5-羟色胺 受体 

分 类 号：R244.9[医药卫生—针灸推拿学]