益气活血生肌方对难愈性创面愈合及血管再生的影响  

Effect of Yiqi Huoxue Shengji Prescription on refractory wound healing and vessel regeneration

作　　者：骆渊城 朱滨 杨威 王奎 LUO Yuancheng;ZHU Bin;YANG Wei;WANG Kui(Department of Orthopaedics and Traumatology,Ethnic Hospital of Enshi Tujia and Miao Autonomous Prefecture,Hubei Province,Enshi445000,China)

机构地区：[1]湖北省恩施土家族苗族自治州民族医院骨伤科,湖北恩施445000

出　　处：《中国医药导报》2023年第12期30-34,共5页China Medical Herald

基　　金：湖北省卫生健康委员会中医药科研项目(ZY2021Q022)。

摘　　要：目的探讨益气活血生肌方对糖尿病大鼠溃疡创面愈合及血管再生的影响。方法按照随机数字表法将60只SPF级体重281~320 g,8~10周龄的雄性SD大鼠分成给药组、难愈性创面组、正常创面组、四君子汤组,每组15只。其中,给药组、难愈性创面组及四君子汤组采用链脲佐菌素法制作成糖尿病模型,48 h后空腹血糖>16.7 mmol/L,出现多尿、多饮等情况,提示造模成功。所有大鼠均于背部制作皮肤缺损创面,给药组及四君子汤组分别用3 ml益气活血生肌方及四君子汤灌胃,其他组用3 ml双蒸水灌胃,早晚各一次。于术后的第5、10、20天每组随机取5只大鼠,测量创面大小,计算创面愈合率;采用苏木精-伊红染色观察并计算溃疡创面毛细血管的数量,采用酶联免疫吸附试验法检测大鼠血液中血管内皮生长因子(VEGF)的含量,采用Western blot法检测创面局部组织VEGF、转化生长因子-β1(TGF-β1)和Smad 3的含量。结果术后第20天各组创面愈合率、血清VEGF表达水平、创面局部组织VEGF蛋白表达水平高于术后第5、10天,术后第10天各组创面愈合率、血清VEGF表达水平、创面局部组织VEGF蛋白表达水平高于本组术后第5天(P<0.05)。术后第20天给药组和正常创面组TGF-β1和Smad3蛋白表达水平低于术后第5、10天,术后第10天给药组和正常创面组TGF-β1和Smad3蛋白表达水平低于术后第5天(P<0.05),术后第20天难愈性创面组TGF-β1蛋白表达水平低于术后第5、10天(P<0.05)。术后第10、20天难愈性创面组Smad3蛋白表达水平高于术后第5天(P<0.05)。术后第5天,难愈性创面组创面愈合率低于正常创面组(P<0.05)。术后第10、20天,难愈性创面组创面愈合率低于正常创面组,给药组创面愈合率高于难愈性创面组(P<0.05)。术后各时间点难愈性创面组血清VEGF表达水平、创面局部组织VEGF蛋白表达水平低于正常创面组,给药组血清VEGF表达水平、创面局�Objective To investigate the effect of Yiqi Huoxue Shengji Prescription on ulcer wound healing and vessel regeneration in diabetic mellitus rats.Methods Sixty male SD rats with SPF grade 281-320 g and age of eight-ten weeks were divided into drug administration group,refractory wound group,normal wound group,and Sijunzi Decoction group according to random number table method,with 15 rats in each group.Among them,the drug administration group,refractory wound group,and Sijunzi Decoction group were prepared into diabetes mellitus model by streptozutocin method,fasting blood glucose>16.7 mmol/L after 48 h,with the occurrence of polyuria and polydipsia,indicating successful modeling.Skin defect wounds were made on the back of all rats.The drug administration group and Sijunzi Decoction group were given 3 ml Yiqi Huoxue Shengji Prescription and Sijunzi Decoction by gavage,respectively,and the other groups were given 3 ml double steaming water by gavage,once in the morning and once in the evening.On the 5th,10th,and 20th day after surgery,five rats in each group were selected to measure the wound size and calculate the wound healing rate.Hematoxylin-eosin staining was used to observe and calculate the number of capillaries on the ulcer wound.The content of vascular endothelial growth factor(VEGF)in the blood of rats was detected by enzyme-linked immunoadsordent assay.The content of VEGF,transforming growth factor-β1(TGF-β1),and Smad3 in the local tissue of the wound was detected by Western blot.Results Wound healing rate,serum VEGF expression level,and VEGF protein expression level in local tissue of wound in each group on the 20th day after surgery were higher than those on the 5th and 10th day after surgery;wound healing rate,serum VEGF expression level,and VEGF protein expression level in local tissue of wound in each group on the 10th day after surgery were higher than those on the 5th day after surgery(P<0.05).The protein expression levels of TGF-β1 and Smad3 in drug administration group and normal wound group

关 键 词：难愈性溃疡 血管内皮生长因子 血管生成 创面愈合 

分 类 号：R285.5[医药卫生—中药学]