海博麦布中对甲苯亚磺酸甲酯、对甲苯亚磺酸乙酯、对甲苯亚磺酸异丙酯残留研究  

Residual research of methyl p-toluene sulfinate,ethyl p-toluene sulfinate and isopropyl p-toluene sulfinate in hybutimibe

作　　者：周雪飞 路昭 戴露媚 丁安平 卢静静 和燕玲 郑晓鹤 张辉 邵伍军 ZHOU Xuefei;LU Zhao;DAI Lumei;Ding Anping;LU Jingjing;HE Yanling;ZHENG Xiaohe;ZHANG Hui;SHAO Wujun(Zhejiang Hisun Pharmaceutical Co.,LTD.,Taizhou Zhejiang 318000,China;Taizhou Economic Construction Planning Institute Co.,LTD.,Taizhou Zhejiang 318000,China;Taizhou Vocational College of Science&Technology,Taizhou Zhejiang 318000,China)

机构地区：[1]浙江海正药业股份有限公司,浙江台州318000 [2]浙江省台州市经济建设规划有限公司,浙江台州318000 [3]台州科技职业学院,浙江台州318000

出　　处：《中兽医医药杂志》2023年第2期56-61,共6页Journal of Traditional Chinese Veterinary Medicine

基　　金：浙江省公益技术应用研究资助项目;台州市科技计划项目(LGN22B020002)。

摘　　要：基于“杂质谱”概念,确立科学杂质研究策略,建立超高效液相色谱-三重四级杆质谱(UPLC-MS/MS)测定海博麦布中基因毒性杂质对甲苯亚磺酸甲酯、对甲苯亚磺酸乙酯、对甲苯亚磺酸异丙酯含量的方法。采用Agilent Eclipse Plus C_(18)色谱柱进行分离,以0.01 mol/L甲酸铵溶液为流动相A,甲醇为流动相B,梯度洗脱,质谱检测器,流速为0.4 mL/min。结果显示,对甲苯亚磺酸甲酯、对甲苯亚磺酸乙酯、对甲苯亚磺酸异丙酯的线性范围均为1.30~10.20 ng/mL,相关系数r均大于0.9990,方法检出限浓度均为0.80 ng/mL,定量限浓度均为2.60 ng/mL,样品加标回收率均为93.48%~112.82%。本方法可以快速、简便、灵敏、准确地定量测定海博麦布中对甲苯亚磺酸甲酯、对甲苯亚磺酸乙酯、对甲苯亚磺酸异丙酯杂质含量。Based on the concept of impurity profile,a scientific research strategy for impurity was established to determine the content of methyl p-toluene sulfinate,ethyl p-toluene sulfinate and isopropyl p-toluene sulfinatein in hybutimibe by ultra performance liquid chromatography-triple quadrupole mass spectrometer.The determination was performed on an agilent eclipse plus C_(18) column with the mobile phase consisting of 0.01 mol/L ammonium formate(phase A)and methanol(phase B)by gradient elution.The detector was mass spectrum.The flow rate was 0.4 mL/min.The results showed that the linear range of three compounds was 1.30-10.20 ng/mL with the correlation coefficients all higher than 0.9990.The limit of detection was 0.80 ng/mL,and the limit of quantification was 2.60 ng/mL.The recoveries were between 93.48%and 112.82%.The method is rapid,simple,sensitive and accurate for the quantitative determination of mthyl ptoluene slfinate,ethyl p-toluene sulfinate and isopropyl p-toluene sulfinate in hybutimibe.

关 键 词：杂质谱 海博麦布 基因毒性 对甲苯亚磺酸甲酯 对甲苯亚磺酸乙酯 对甲苯亚磺酸异丙酯 超高效液相色谱-三重四级杆质谱 

分 类 号：S859.79[农业科学—临床兽医学]