5-氯水杨醛-罗丹明B荧光探针的合成及其识别性能研究  

Synthesis and Recognition of 5-Chlorosalicylaldehyde-Rhodamine B Fluorescent Probe

作　　者：刘晓作 温小洪 洪利明 祝显虹 Liu Xiaozuo;Wen Xiaohong;JHong Liming;Zhu Xianhong(College of Chemistry and Environmental Science,Shangrao Normal University,Shangrao,Jiangi33400l,China)

机构地区：[1]上饶师范学院化学与环境科学学院,江西上饶334001

出　　处：《化学世界》2023年第2期123-128,共6页Chemical World

基　　金：江西省教育厅科技(No.201721);上饶师范学院科研(No.201947);上饶市基础应用研究(No.SKB2021018)资助项目。

摘　　要：以水合肼、5-氯水杨醛和罗丹明B为原料,合成了5-氯水杨醛-罗丹明类衍生物探针L,目标化合物5-氯水杨醛罗丹明B联肼经核磁、红外光谱和质谱进行了表征,采用紫外光谱法和荧光光谱法研究了其与金属离子的识别性能。结果表明,该探针可选择性紫外识别Cu^(2+)和荧光识别Hg^(2+),在1.0~12.0μmol·L^(-1)范围定量检测Cu^(2+),R=0.9986,检出限为0.53μmol·L^(-1);在3.0~7.0μmol·L^(-1)范围定量检测Hg^(2+),580 nm处的荧光强度与Hg^(2+)浓度呈良好的线性关系,相关系数(R)=0.9974,检出限为0.56μmol·L^(-1)。Job’s曲线表明,探针L与Cu^(2+)、探针L与Hg^(2+)的结合物质的量的比均为1∶1,并对其响应机理进行了探讨。5-Chlorosalicylaldehyde-rhodamine B fluorescent probe L was synthesized from rhodamine B,hydrazine hydrate and 5-chlorosalicylaldehyde.The structure of 5-chlorosalicylaldehyde-rhodamine B hydrazine was characterized by^(1)H NMR,IR and MS.The recognition properties of probe with metal ions have been investigated by UV/Vis and fluorescence spectrophotometry.The results showed that the probe can exhibit the selective colorimetric recognition of Cu^(2+)and fluorogentic recognition of Hg^(2+),while the concentration of Cu^(2+)is between 1.0~12.0μmol.L^(-1)with a linear correlation coefficient of(R)=0.9986 and a detection limit of 0.53μmol·L^(-1).The fluorescent intensity at 580 nm exhibited a good linear relationship with the concentration of Hg^(2+)between 3.0 to 7.0μmol·L^(-1)with a linear correlation coefficient of(R)=0.9974 and a detection limit of 0.56μmol L^(-1).The Job's plot confirmed the 1:1 complex between Cu^(2+),Hg?+and probe,and the recognition mechanism was proposed.

关 键 词：罗丹明B 荧光探针 响应机理 5-氯水杨醛 

分 类 号：O623.5[理学—有机化学]