Single-cell sequencing analysis reveals the molecular mechanism of promotion of SCAP proliferation upon AZD2858 treatment  

作　　者：YIFAN XU DONGMEI CHENG LEI HU XIN DONG LIYING LV CHEN ZHANG JIAN ZHOU 

机构地区：[1]Beijing Laboratory of Oral Health,School of Stomatology,Capital Medical University,Beijing,100050,China [2]Department of Neurobiology,School of Basic Medical Sciences,Capital Medical University,Beijing,100069,China [3]Department of VIP Dental Service,School of Stomatology,Capital Medical University,Beijing,100050,China [4]Laboratory for Oral and General Health Integration and Translation,Beijing Tian Tan Hospital,Capital Medical University,Beijing,100070,China

出　　处：《BIOCELL》2023年第4期825-836,共12页生物细胞（英文）

基　　金：the fund of National Natural Science Foundation of China(82170951);Beijing Natural Science Foundation(7222079).

摘　　要：The Wnt/β-catenin signaling pathway is the main target of tooth regeneration regulation.Treatment of cells with AZD2858 stimulates the Wnt/β-catenin signaling pathway,yet the function of this pathway in tooth regeneration remains unclear.Here,we found that AZD2858 promotes the accumulation ofβ-catenin in the nuclei of stem cells from the apical papilla(SCAPs)and enhances cell proliferation.Single-cell sequencing was performed on SCAPs treated with AZD2858.Eight clusters were identified,namely SCAPs-CNTNAP2,SCAPs-DTL,SCAPs-MYH11,SCAPs-MKI67,SCAPs-CXCL8,SCAPs-TPM2,SCAPs-IFIT2 and SCAPs-NEK10.The pseudo-time trajectory analysis showed that AZD2858 enhanced the evolution of SCAPs from SCAPs-TMP2 clusters to SCAPs-MYH11,SCAPs-CNTNAPs and SCAPs-NEK10 clusters via up-regulation of PRKCA,SMURF2,MAGI2,RBMS3,EXT1,CAMK2D,PLCB4,and PLCB1.These results demonstrate that AZD2858 enhances the proliferation of SCAPs-TPM2 cluster by activating the non-canonical Wnt/β-catenin signaling pathway.

关 键 词：AZD2858 Cell proliferation Stem cells Single-cell sequencing Wnt/β-catenin signaling pathway 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]