二恶英暴露下奶牛血液代谢组学研究  

Metabolomics study on blood of cow exposed to PCDD/Fs

作　　者：胡晓旭 许彤 陈旸升 谢群慧[1,4] 郝艳芬 王楚 程劼 王璞[3] 张庆华[1,4] 徐丽[1,4] 赵斌 HU Xiaoxu;XU Tong;CHEN Yangsheng;XIE Qunhui;HAO Yanfen;WANG Chu;CHEN Jie;WANG Pu;ZHANG Qinghua;XU Li;ZHAO Bin(State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing,100085,China;Institute of Quality Standards and Testing Technology for AgroProducts,Chinese Academy of Agricultural Sciences,Beijing,100081,China;Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances,Institute of Environment and Health,Jianghan University,Wuhan,430056,China;University of Chinese Academy of Sciences,Beijing,100049,China)

机构地区：[1]中国科学院生态环境研究中心,环境化学与生态毒理学国家重点实验室,北京100085 [2]中国农业科学院农业质量标准与检测技术研究所,北京100081 [3]江汉大学持久性有毒污染物环境与健康危害湖北省重点实验室,环境与健康学院,武汉430056 [4]中国科学院大学,北京100049

出　　处：《环境化学》2023年第4期1067-1076,共10页Environmental Chemistry

基　　金：国家重点研发计划(2017YFC1600301);国家自然科学基金(22076216,21836004)资助.

摘　　要：旨在研究食用被PCDD/Fs污染的饲料对奶牛血液代谢组的影响.选择飞灰作为PCDD/Fs的载体,暴露期间将30 g飞灰与40 kg奶牛饲料均匀混合,使用配制的污染饲料饲喂奶牛,连续饲喂38 d后采集血液.采用液相色谱-质谱(LC-MS)联用技术对血液进行代谢组学分析,并且结合主成分分析(PCA)和正交偏最小二乘法判别分析(OPLS-DA)对代谢轮廓进行模式识别分析并筛选差异代谢产物.共鉴定差异代谢物50个,主要为氨基酸、胆酸、磷脂与脂肪酸类成分.将差异代谢物进行通路分析并注释,涉及19条代谢通路,主要与氨基酸、脂肪酸及胆碱代谢相关.PCDD/Fs暴露干扰了奶牛的脂肪酸代谢、氨基酸代谢、磷脂代谢、胆汁酸代谢,可作为PCDD/Fs暴露的靶标代谢通路供进一步的研究.The aim of this study was to explore the influence of PCDD/Fs pollution on the blood metabolism of cows.Fly ash was selected as the carrier of PCDD/Fs,30 g of fly ash and 40 kg of cow feed were homogeneously mixed during exposure,the prepared polluted feed is used for feeding cows,and blood is collected after continuous feeding for 38 days.Using liquid chromatography-mass spectrometry(LC-MS)technology to analyze blood metabolism,principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)were combined to conduct pattern recognition analysis on metabolic profiles and screen differential metabolites.A total of 50 different metabolites were identified,mainly amino acids,bile acids,phospholipids,and fatty acids.Differential metabolites were subjected to pathway analysis and noted.There were 19 metabolic pathways involved,mainly related to the metabolism of amino acids,fatty acids and choline.In summary,the results showed that PCDD/Fs exposure interfered with fatty acid metabolism,amino acid metabolism,phospholipid metabolism and bile acid metabolism of cows,and it could be used as the target metabolic pathway of PCDD/Fs exposure for further research.

关 键 词：二恶英 奶牛 代谢组学 LC-MS 

分 类 号：X503.221[环境科学与工程—环境工程] S823[农业科学—畜牧学]