过饱和度、钙/草酸化学计量比对草酸钙晶体形成的影响及降解茯苓多糖的调控作用  

Effects of supersaturation,Ca^(2+)/C_(2)O_(4)^(2-)stoichiometric ratio on calcium oxalate crystallization and the regulation of degraded Poria cocos polysaccharide

作　　者：郑雨云 刘静鸿 陈佳云 欧阳健明 ZHENG Yu-Yun;LIU Jing-Hong;CHEN Jia-Yun;OUYANG Jian-Ming(College of Chemistry and Materials Science,Institute of Biomineralization and Lithiasis Research,Jinan University,Guangzhou 510632,China)

机构地区：[1]暨南大学化学与材料学院,生物矿化与结石病防治研究所,广州510632

出　　处：《无机化学学报》2023年第5期794-804,共11页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.21975105)资助。

摘　　要：为研究草酸钙(CaC_(2)O_(4))晶体成核、生长和聚集,探讨降解茯苓多糖(PCP)的抑制作用,采用X射线衍射、FT-IR、扫描电镜、拉曼光谱、ζ电位仪和紫外分光光度计等方法对不同条件下形成的CaC_(2)O_(4)晶体进行表征。结果表明,在低过饱和度(RS≤26.6)时,主要生成一水草酸钙(COM)晶体;至RS为37.6和46.0时分别生成了11.6%和38.3%的二水草酸钙(COD)晶体,且高RS时晶体的聚集程度增加。在RS相同时,随着Ca^(2+)/Ox^(2-)(Ox^(2-)=C_(2)O_(4)^(2-))化学计量比(n_(Ca^(2+))/n_(Ox^(2-)))增大,晶体中COD比例增加。降解PCP的加入可增加体系中可溶性Ca^(2+)浓度,减少生成的CaC_(2)O_(4)晶体质量,增加晶体表面ζ电位绝对值,这些均有利于抑制CaC_(2)O_(4)结石的形成。因此,高Ox^(2-)浓度对肾结石形成的风险远大于高Ca^(2+)浓度,提示草酸的摄入对CaC_(2)O_(4)结石的风险远远大于钙的摄入,降解PCP能同时抑制CaC_(2)O_(4)晶体的成核、生长和聚集。To study the nucleation,growth,and aggregation of calcium oxalate(CaC_(2)O_(4))crystals,and to explore the inhibitory effect of degraded Poria cocos polysaccharide(PCP),CaC_(2)O_(4) crystals formed under different conditions were characterized by X-ray diffraction,FT-IR,scanning electron microscope,Raman spectroscopy,ζpotentiometer,and UV spectrophotometer.The results showed that calcium oxalate monohydrate(COM)crystals were mainly formed at low supersaturation(RS≤26.6).At RS being 37.6 and 46.0,11.6%and 38.3%calcium oxalate dihydrate(COD)crystals were formed respectively,and the aggregation degree of the crystals increased at high RS.At the same RS,the proportion of COD in the crystal increased with the increase of the Ca^(2+)/Ox^(2-)stoichiometric ratio(n_(Ca^(2+))/n_(Ox^(2-)),Ox^(2-)=C_(2)O_(4)^(2-)).The addition of degraded PCP could increase the concentration of soluble Ca^(2+)ions in the system,reduce the quality of generated CaC_(2)O_(4) crystals,and increase the absolute value ofζpotential on the crystal surface,all of which are beneficial to inhibit the formation of CaC_(2)O_(4) stones.Thus,the risk of high Ox^(2-)concentration on the formation of kidney stones is much greater than that of high Ca^(2+)concentration,suggesting that the risk of oxalic acid intake on CaC_(2)O_(4) stones is much greater than that of calcium intake.PCP can simultaneously inhibit the nucleation,growth,and aggregation of CaC_(2)O_(4) crystals.

关 键 词：草酸钙 茯苓多糖 Ca^(2+)/Ox^(2-)化学计量比 过饱和度 晶体调控 

分 类 号：R69[医药卫生—泌尿科学] O614.23[医药卫生—外科学]