PEG-6000模拟干旱胁迫下5份苜蓿材料萌发期抗旱性鉴定与比较  

Characterization and Comparison of Drought Resistance of Five Alfalfa Varieties during Germination Period under PEG-6000 Simulated Drought Stress

作　　者：王江银 徐婉宁 苏洋[1] 张博[1] WANG Jiangyin;XU Wanning;SU Yang;ZHANG Bo(College of Grassland Science,Xinjiang Agricultural University/Xinjiang Key Laboratory of Grassland Resources and Ecology,Urumqi 830052,China)

机构地区：[1]新疆农业大学草业学院/新疆草地资源与生态重点实验室,新疆乌鲁木齐830052

出　　处：《畜牧与饲料科学》2023年第2期81-91,共11页Animal Husbandry and Feed Science

基　　金：农业专项课题——国家牧草产业技术体系项目(CARS-34)。

摘　　要：[目的]分析国内外不同品种苜蓿材料萌发期的抗旱性,筛选出抗旱性较强的苜蓿品种。[方法]分别采用浓度为0、5%、10%、15%、20%的聚乙二醇-6000(PEG-6000)溶液对5份苜蓿种质材料(新牧4号、中苜3号、歌纳、A-17、D-23)进行模拟干旱胁迫处理,以蒸馏水处理为对照组(CK);测定不同品种苜蓿种质材料在不同PEG-6000处理浓度下萌发期的发芽指标及抗旱系数,利用隶属函数法对其抗旱性能进行综合评价。[结果]通过测定种子萌发的相关指标以及利用隶属函数法对不同萌发指标进行多维度综合评价,不同苜蓿材料种子萌发期的抗旱性强弱顺序依次为歌纳>A-17>新牧4号>D-23>中苜3号。[结论]在PEG-6000模拟自然干旱的试验条件下,综合抗旱性最好的苜蓿品种为歌纳,其次是A-17。[Objective]This study was conducted to characterize the drought resistance of alfalfa varieties domestically bred in China and introduced from abroad during germination period,and to select the alfalfa varieties with strong drought resistance.[Method]Polyethylene glycol 6000(PEG-6000)solutions were used to simulate drought stress on five alfalfa germplasm materials(Xinmu No.4,Zhongmu No.3,Gena,A-17,D-23)at concentrations of 0,5%,10%,15%and 20%,respectively.The control group(CK)was treated with distilled water.The germination indices and drought resistance coefficient of the alfalfa germplasm materials at different PEG-6000 treatment concentrations during germination period were measured,and their drought resistance performance was comprehensively assessed using the membership function method.[Result]The analysis of germination indices as well as the multi-dimensionally and comprehensively evaluation on germination indices by the membership function method demonstrated that Gena had the highest drought resistance during germination period among the tested alfalfa varieties,followed by A-17,Xinmu No.4,D-23 and Zhongmu No.3.[Conclusion]Under PEG-6000 simulated drought conditions,the alfalfa variety with the best comprehensive drought resistance was Gona,followed by A-17.

关 键 词：苜蓿 种子萌发 抗旱性 隶属函数 PEG-6000 

分 类 号：Q945.78[生物学—植物学] S541[农业科学—作物学]