加味补中益气汤对COPD模型大鼠肠道菌群的影响  

Effects of modified Buzhong Yiqi Decoction on intestinal microflora in a rat model of chronic obstructive pulmonary disease

作　　者：胡旭贞 洪波 周晶晶 Hu Xuzhen;Hong Bo;Zhou Jingjing(Department of Respiration,Ningbo Municipal Hospital of Traditional Chinese Medicine,Ningbo 315000,Zhejiang Province,China)

机构地区：[1]宁波市中医院呼吸科,宁波315000

出　　处：《中国基层医药》2023年第4期570-576,共7页Chinese Journal of Primary Medicine and Pharmacy

基　　金：浙江省宁波市科技计划(2019A610365)。

摘　　要：目的分析加味补中益气汤对慢性阻塞性肺疾病(COPD)模型大鼠肠道菌群的影响。方法2021年4-6月,选取SPF级Wistar大鼠60只,采用随机数字表法分为空白对照(BC)组、模型(Model)组、中药(CM)组、西药(WM)组,每组15只。除BC组外均以烟熏法建立肺脾两虚型COPD模型,造模后CM组、WM组分别以加味补中益气汤、合生元灌胃干预,Model组、BC组则以0.9%氯化钠注射液灌胃干预。7 d后收集各组大鼠粪便进行肠道菌群16S rRNA测序,对有效序列进行聚类,获得操作分类单元(OTU),用于主坐标分析(PCoA)、物种组成分析、Alpha多样性分析,分析加味补中益气汤对肠道菌群结构、多样性和丰度变化的影响。结果CM组和WM组以厚壁菌门为主,BC组和Model组以拟杆菌属为主。各组中优势菌群主要为乳杆菌、拟杆菌属。Alpha多样性分析显示,CM组、WM组、BC组群落丰富度指数中Shannon指数分别为(3.65±0.35)、(3.65±0.36)、(3.59±0.20),均高于Mode组的(3.37±0.26)(t=2.49、2.44、2.60,均P<0.05),CM组、WM组、BC组Shannon指数差异无统计学意义(P>0.05);CM组、WM组、BC组Sobs指数分别为(458.67±73.11)、(454.80±95.13)、(525.93±101.88),均高于Mode组的(337.40±37.49)(t=5.72、4.45、6.73,均P<0.05),且BC组Sobs指数高于WM组,BC组与CM组、WM组与CM组Sobs指数比较,差异均无统计学意义(均P>0.05)。PCoA分析显示,与BC组比较,门水平上Model组放线菌门下降、变形菌门和脱硫菌门上升,属水平上Model组拟杆菌属、罗姆布茨菌属、毛螺杆菌属及副拟杆菌属上升,普雷沃菌属、梭菌属、布氏菌属和瘤胃球菌下降;与WM组相比,CM组杆菌属、普雷沃菌属、布氏菌属和拟普雷沃菌属上升,梭菌属、嗜果胶拟杆菌属、克里斯滕森菌属和毛螺杆菌属下降,各组菌群组成差异均有统计学意义(均P<0.05)。结论COPD模型大鼠存在肠道微生态失衡,加味补中益气汤可调节肠道微生态环境,改善肠道菌群结构Objective To investigate the effects of modified Buzhong Yiqi Decoction on intestinal microflora in a rat model of chronic obstructive pulmonary disease.Methods From April to June 2021,60 specific pathogen-free Wistar rats were selected for this study.They were randomly divided into blank control,model,traditional Chinese medicine,and western medicine groups with 15 rats per group.Rat models of chronic obstructive pulmonary disease with lung and spleen deficiency were established in all groups except the blank control group.Rat models in the traditional Chinese medicine and western medicine groups were administered modified Buzhong Yiqi Decoction and synbiotics.Rat models in the model and blank control groups were identically administered 0.9%sodium chloride injection.After 7 days,the feces of rats in each group were collected for 16S rRNA sequencing of intestinal flora.Effective sequences were clustered to obtain operational taxonomic units for principal coordinate analysis,species composition analysis,and alpha diversity analysis.The effects of modified Buzhong Yiqi Decoction on the structure,diversity,and abundance changes of intestinal flora were analyzed.Results The dominant bacteria in the traditional Chinese medicine and western medicine groups were Firmicutes,while the dominant bacteria in the blank control and model groups were Bacteroides.The dominant bacterial groups in each group were mainly Lactobacillus and Bacteroides.Alpha diversity analysis showed that the Shannon index in the community diversity indices of traditional Chinese medicine,western medicine,and blank control groups was(3.65±0.35),(3.65±0.36),and(3.59±0.20),respectively,which were significantly higher than(3.37±0.26)in the model group(t=2.49,2.44,2.60,all P<0.05).There was no significant difference in the Shannon index among traditional Chinese medicine,western medicine,and blank control groups(all P>0.05).The Sobs index of the traditional Chinese medicine,western medicine,and blank control group was(458.67±73.11),(454.80±95.13),

关 键 词：补中益气汤 肺疾病 慢性阻塞性 疾病模型 动物 大鼠 肠道菌群 高通量核苷酸序列分析 结构 多样性 丰度 

分 类 号：R285.5[医药卫生—中药学]