东乡族2型糖尿病患者与健康人群肠道菌群构成特点及差异的研究  

Gut microbiome characteristics in patients with type 2 diabetes mellitus of Dongxiang Group in Gansu province

作　　者：马晓兰 侯彦军 马志明 胡雪剑[1] 葛丹[1] Ma Xiaolan;Hou Yanjun;Ma Zhiming;Hu Xuejian;Ge Dan(Department of Endocrinology and Metabolism,the Second Hospital of Lanzhou University,Lanzhou 730000,China;Department of General Medicine,People′s Hospital of Dongxiang County,Dongxiang 731400,China;Department of Emergency,Integrated Traditional Chinese and Western Medicine Hospital of Guanghe County,Guanghe 731300,China)

机构地区：[1]兰州大学第二医院内分泌代谢科,兰州730000 [2]东乡县人民医院综合内科,东乡731400 [3]广河县中西医结合医院急诊科,广河731300

出　　处：《中国医师进修杂志》2023年第4期305-310,共6页Chinese Journal of Postgraduates of Medicine

摘　　要：目的探究东乡族2型糖尿病与健康人群肠道菌群的特点及差异。方法收集2020年4月至2021年1月就诊于东乡县人民医院的东乡族2型糖尿病患者25例(T2DM组),同时选取28例体检健康的东乡族人群作为对照组(HC组),收集研究对象及HC组的新鲜粪便样本,通过对16S核糖体RNA(16S rRNA)基因V3+V4区扩增子测序的方法分析肠道菌群。初步了解东乡族T2DM组与HC组肠道微生物的构成特点及差异。结果两组样本共检测出936个操作分类单元(OTU)数目,其中共同有OTU数目为633个。Alpha多样性分析中Shannon指数显著高于对照组,Simpson指数显著低于对照组,两组菌群多样性差异有统计学意义(P<0.05)。使用置换多元方差分析比较两组β多样性,差异有统计学意义(P<0.05)。在分类学门水平,T2DM组中厚壁菌门、放线菌门显著高于HC组(37.97%比22.89%、5.09%比2.08%),差异有统计学意义(P<0.05);而拟杆菌门丰度显著减少(T2DM组:68.00%,HC组:49.75%),差异有统计学意义(P<0.05)。在属水平,发现20个菌属差异有统计学意义(P<0.05),T2DM组中双歧杆菌属、大肠埃希-志贺菌属、Tyzzerella_4的丰度显著高于HC组,而Prevotella_9、罗氏菌属、Erysipelotrichaceae_UCG-003菌显著减少。结论东乡族T2DM组和健康人群肠道菌群组成存在显著差异,从而初步了解东乡族T2DM患者的肠道菌群特征,为今后治疗提供依据。Objective To investigate the region-specific characteristics of the gut microbiota and evaluate the association of specific gut microbes with type 2 diabetes mellitus(T2DM)from the Dongxiang Group in Gansu province,Northwest China.Methods Fifty-three participants who was born in Dongxiang Autonomous County(Gansu Province)from April 2020 to January 2021 were enrolled,including 25 patients with T2DM recruited from the outpatient departments of internal medicine at The People′s Hospital of Dongxiang County(T2DM group)and 28 healthy controls recruited from the health screening center(HC group).Gut microbiome composition was analyzed using a 16S ribosomal RNA gene-based sequencing protocol.Results A total of 936 operational taxonomic units(OTU)were obtained in the two groups.Of note,the HC and T2DM groups had 633 OTU in common.The alpha and beta diversity were different between the two groups(P<0.05).Shannon index was significantly higher than that in the HC group,and Simpson index was significantly lower than that in the HC group,displacement multivariate analysis of variance was used to compareβdiversity between the two groups,and the difference was statistically significant(P<0.05).At the Phylum level,firmicutes and actinomycetes in T2DM group were significantly higher than those in the HC group(37.97%vs.22.89%,5.09%vs.2.08%),and the differences were statistically significant(P<0.05).The abundance of Bacteroidetes was significantly decreased(68.00%in T2DM group and 49.75%in HC group),and the difference was statistically significant(P<0.05).At the genus level,there were 20 genera statistically significant differences between the two groups.The abundance of Bifidobacterium,Escherichia,Shigella,and Tyzzerella_4 levels were significantly increased in the T2DM group,but Prevotella_9,Erysipelotrichaceae_UCG-003,and Roseburia levels were significantly decreased in the T2DM group compared to those in the HC group.Conclusions There is a significant difference in the gut microbiota between patients with T2DM and healthy

关 键 词：糖尿病 2型 阴沟肠道菌属 东乡族 构成 

分 类 号：R587.1[医药卫生—内分泌]