Functional characterization of sex pheromone receptors in Spodoptera frugiperda, S. exigua, and S. litura moths  

作　　者：Sai Zhang Fang Liu Bin Yang Yang Liu Gui-Rong Wang 

机构地区：[1]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Bejing,China [2]College of Horticulture and Plant Protection,Yangzhou University,Yangzhou,China [3]Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,China

出　　处：《Insect Science》2023年第2期305-320,共16页昆虫科学（英文版）

基　　金：funded byNational Natural Science Foundationof China(32072509,31725023,and 31911530234);National Key Research and Developmentt Program of China(2021YFD1400703);Shenzhen Science and Technology Program(KQTD20180411143628272);Central Public-interest Scientific Institution Basal Research Fund(CAASZDRW202108);projects subsidized by Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District(PT202101-02);Agricultural Science and Technology Innovation Program(ASTIP).

摘　　要：Moths possess an extremely sensitive and diverse sex pheromone processing system,in which pheromone receptors(PRs)are essential to ensure communication between mating partners.Functional properties of some PRs are conserved among species,which is important for reproduction.However,functional differentiation has occurred in some homologous PR genes,which may drive species divergence.Here,using genome analysis,17 PR genes were identified from Spodoptera frugiperda,S.exigua,and S.litura,which belong to 6 homologous groups(odorant receptor[OR]6,11,13,16,56,and 62);of which 6 PR genes(OR6,OR11,OR13,OR16,OR56,and OR62)were identified in S.frugiperda and S.exigua,and 5 PR genes were identified in S.litura,excluding OR62.Using heterologous expression in Xenopus oocytes,we characterized the functions of PR orthologs including OR6,OR56,and OR62,which have not been clarified in previous studies.OR6 orthologs were specifically tuned to(Z,E)-9,12-tetradecadienyl acetate(Z9,E12-14:OAc),and OR62 orthologs were robustly tuned to Z7-12:OAc in S.frugiperda and S.exigua.The optimal ligand for OR56 was Z7-12:OAc in S.frugiperda,but responses were minimal in S.exigua and S.litura.In addition,SfruOR6 was male antennae-specific,whereas SfruOR56 and SfruOR62 were male antennae-biased.Our study further clarified the functional properties of PRs in 3 Spodoptera moth species,providing a comprehensive understanding of the mechanisms of intraspecific communication and interspecific isolation in Spodoptera.

关 键 词：PHEROMONE pheromone receptor SPODOPTERA Xenopus oocytes 

分 类 号：O64[理学—物理化学]