BmMD-2A responds to 20-hydroxyecdysone and regulates Bombyx mori silkworm innate immunity in larva-to-pupa metamorphosis  

作　　者：Ruonan Zhang Xue Chen Yuan Wang Xu Bai Qiong Yang Yangjin Zhong Xiao-Qiang Yu Fengliang Jin Wanying Yang 

机构地区：[1]Guangdong Laboratory for Lingnan Modern Agriculture,Laboratory of Insect Molecular Biology and Biotechnology,Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding,College of Animal Science,South China Agricultural University,Guangzhou,510642,China [2]Guangdong Laboratory of Bio-Pesticide Creation and Application of Guangdong Province,College of Plant Protection,South China Agricultural University,Guangzhou,510642,China [3]The Sericultural and Agri-Food Research Institute of the Guangdong Academy of Agricultural Sciences,Guangzhou,China [4]Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,Institute of Insect Science and Technology,School of Life Sciences,South China Normal University,Guangzhou,China

出　　处：《Insect Science》2023年第2期411-424,共14页昆虫科学（英文版）

基　　金：funded by grants from the National Science Foundation of China(grant no.31970462);the Natural Science Foundation of Guangdong Province(no.2020A1515110805,no.2020A1515010867,no.2021A1515012090).

摘　　要：20E-hydroxyecdysone(20E)plays important roles in larval molting and metamorphosis in insects and is also involved in the insect innate immune response.Insect metamorphosis is a highly successful strategy for environmental adaptation and is the most vulnerable stage during which the insect is susceptible to various pathogens.20E regulates a series of antimicrobial peptides(AMPs)through the immunodeficiency(IMD)pathway activation in Drosophila;nevertheless,whether other immune pathways are involved in 20E-regulated insect immunity is unknown.Our previous studies showed that BmMD-2A is a member of the MD-2-related lipid recognition(ML)family of proteins that are involved in the Bombyx mori innate immunity Toll signaling pathway.In this study,we further demonstrate that BmMD-2A is also positively regulated by 20E,and the BmMD-2A neutralization experiment suggested that 20E activates some downstream immune effect factors,the AMP genes against Escherichia coli and Staphylococcus aureus,through the regulation of BmMD-2A in larval metamorphosis,implying that B.mori may use the Toll-ML signaling pathway to maintain innate immune balance in the larval-pupal metamorphosis stage,which is a different innate immunity pathway regulated by 20E compared to the IMD pathway in Drosophila.

关 键 词：AMPs BmMD-2A 20E innate immune METAMORPHOSIS 

分 类 号：Q96[生物学—昆虫学]