Integrated analysis of miRNA and mRNA expression profiles in response to gut microbiota depletion in the abdomens of female Bactrocera dorsalis  

作　　者：JunfeiXie Zhaohui Cai Wenping Zheng Hongyu Zhang 

机构地区：[1]Key Laboratory of Horticultural Plant Biology(MOE),Hubei Hongshan Laboratory,China-Australia Joint Research Centre for Horticultural and Urban Pests,Institute of Urban and Horticultural Entomology,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,430070,China

出　　处：《Insect Science》2023年第2期443-458,共16页昆虫科学（英文版）

基　　金：supported by the National Key R&D Program of China(No.2019YFD1002100);China Agriculture Research System of MOF and MARA(CARS-26).

摘　　要：Insect gut microbiota has been reported to participate in regulating host multiple biological processes including metabolism and reproduction.However,the corresponding molecular mechanisms remain largely unknown.Recent studies suggest that microRNAs(miRNAs)are involved in complex interactions between the gut microbiota and the host.Here,we used next-generation sequencing technology to characterize miRNA and mRNA expression profiles and construct the miRNA–gene regulatory network in response to gut microbiota depletion in the abdomens of female Bactrocera dorsalis.A total of 3016 differentially expressed genes(DEGs)and 18 differentially expressed miRNAs(DEMs)were identified.Based on the integrated analysis of miRNA and mRNA sequencing data,229 negatively correlated miRNA-gene pairs were identified from the miRNA-mRNA network.Gene ontology enrichment analysis indicated that DEMs could target several genes involved in the metabolic process,oxidation-reduction process,oogenesis,and insulin signaling pathway.Finally,real-time quantitative polymerase chain reaction further verified the accuracy of RNA sequencing results.In conclusion,our study provides the profiles of miRNA and mRNA expressions under antibiotics treatment and provides an insight into the roles of miRNAs and their target genes in the interaction between the gut microbiota and its host.

关 键 词：Bactrocera dorsalis gut microbiota microRNA-mRNA regulatory pathways 

分 类 号：Q96[生物学—昆虫学]