Evolutionary genomics analysis reveals gene expansion and functional diversity of arylalkylamine N-acetyltransferases in the Culicinae subfamily of mosquitoes  

作　　者：Yu Tang Huaqing Chen Zhinan Lin Lei Zhang Archana Upadhyay Chenghong Liao David J.Merkler Qian Han 

机构地区：[1]Laboratory of Tropical Veterinary Medicine and Vector Biology,School of Life Sciences,Hainan University,Haikou,China [2]One Health Institute,Hainan University,Haikou,China [3]Department of Chemistry,University of South Florida,Tampa,Florida,USA

出　　处：《Insect Science》2023年第2期569-581,共13页昆虫科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(31860702 and 31960703);by the Hainan Province Science and Technology Special Fund(ZDKJ2021035).

摘　　要：Arylalkylamine N-acetyltransferase(aaNAT),considered a potential new insecticide target,catalyzes the acetylation of arylalkylamine substrates such as serotonin and dopamine and,hence,mediates diverse functions in insects.However,the origin of insect aaNATs(iaaNATs)and the evolutionary process that generates multiple aaNATs in mosquitoes remain largely unknown.Here,we have analyzed the genomes of 33 species to explore and expand our understanding of the molecular evolution of this gene family in detail.We show that aaNAT orthologs are present in Bacteria,Cephalochordata,Chondrichthyes,Cnidaria,Crustacea,Mammalia,Placozoa,and Teleoste,as well as those from a number of insects,but are absent in some species of Annelida,Echinozoa,and Mollusca as well as Arachnida.Particularly,more than 10 aaNATs were detected in the Culicinae subfamily of mosquitoes.Molecular evolutionary analysis of aaNAT/aaNAT-like genes in mosquitoes reveals that tandem duplication events led to gene expansion in the Culicinae subfamily of mosquitoes more than 190 million years ago.Further selection analysis demonstrates that mosquito aaNATs evolved under strongly positive pressures that generated functional diversity following gene duplication events.Overall,this study may provide novel insights into the molecular evolution of the aaNAT family in mosquitoes.

关 键 词：arylalkylamine N-acetyltransferase functional diversity gene duplication molecular evolution MOSQUITO N-acyltransferase 

分 类 号：Q78[生物学—分子生物学]