Plackett-Burman结合Box-Behnken响应面法优化赪桐中类叶升麻苷纯化工艺及其稳定性分析  

Optimization of purification process and stability of acteoside from Clerodendrum japonicum by Plackett-Burman combined with Box-Behnken response surface method

作　　者：张雅昆 王亭亭 张洪艳 霍晓敏 薛站 曾艳荣 谭承建[1] ZHANG Yakun;WANG Tingting;ZHANG Hongyan;HUO Xiaomin;XUE Zhan;ZENG Yanrong;TAN Chengjian(College of ethnic medicine,Guizhou Minzu University,Guiyang,Guizhou Province 550025,China;College of chemical engineering,Guizhou Minzu University,Guiyang,Guizhou Province 550025,China)

机构地区：[1]贵州民族大学民族医药学院,贵州贵阳550025 [2]贵州民族大学化学工程学院,贵州贵阳550025

出　　处：《中国饲料》2023年第9期38-44,共7页China Feed

基　　金：国家自然科学基金项目(31360084,31660103,32160110);贵州省科技计划项目(黔科合支撑[2019]2414)。

摘　　要：为优化赪桐中类叶升麻苷的纯化工艺,并分析其稳定性,试验对8种不同型号的大孔树脂进行吸附-解吸能力比较,筛选出最优的大孔树脂型号;采用Plackett-Burman结合Box-Behnken响应面法,考察上样液浓度、上样液流速、洗脱液浓度等因素水平,优化赪桐中类叶升麻苷的纯化工艺。通过高效液相测定纯化物在不同温度、光照、pH、抗氧化剂等环境条件下类叶升麻苷的含量。结果表明:赪桐中类叶升麻苷最佳纯化工艺条件为ADS-7型号大孔吸附树脂、上样液浓度9.0 mg/mL、上样液流速3.0 m L/min、洗脱液浓度58%,验证试验中的类叶升麻苷回收率可达到76.04%,与所建模型预测接近;稳定性试验表明,温度越高、光照时间越久、pH越高、类叶升麻苷的稳定性越差。同时,添加抗氧化剂可以明显增强其稳定性。故纯化后的类叶升麻苷应在低温、避光、酸性环境中贮藏和使用,且可通过添加不同种抗氧化剂延长其使用期限。采用Plackett-Burman结合Box-Behnken响应面法优化赪桐中类叶升麻苷纯化工艺稳定可行,可为后续抗奶牛乳房炎中草药型饲料添加剂产品的研发及产品稳定性使用提供理论依据。In order to optimize the purification process of acteoside in Clerodendrum japonicum,and analyze its stability,eight kinds of macroporous resin adsorption-desorption capacity of test,screen out the best macroporous resin model;Plackett-burman and Box-Behnken response surface method were used to investigate the concentration of loading liquid,the flow rate of loading liquid,the concentration of eluent and other factors to optimize the purification process of acteoside from Clerodendrum japonicum.The relative content of the purified substance was determined by HPLC under different temperature,light,pH and antioxidant conditions.The results showed that the optimal purification conditions of acteoside from Clerodendrum japonicum were as follows:ADS-7 macroporous adsorption resin,the concentration of loading liquid was 9.0 mg/mL,the flow rate of loading liquid was 3.0 mL/min,and the eluent concentration was 58%.The recovery rate of acteoside in the verification test was 76.04%,which was close to the prediction of the model.The stability study showed that the higher temperature,the longer illumination time,and the higher pH,the worse stability of acteoside.At the same time,adding antioxidant can significantly enhance its stability.Therefore,the purified acteoside should be stored and used in low temperature,dark and acidic environment,and its service life could be extended by adding different kinds of antioxidant.Plackett-burman combined with Box-Behnken response surface method was used to optimize the purification process of acteoside of Clerodendrum japonicum,which was stable and feasible,and could provide theoretical basis for the research and development of the Chinese herbal feed additives and the stable use of the product.

关 键 词：赪桐 类叶升麻苷 纯化工艺 稳定性 

分 类 号：S816.5[农业科学—饲料科学]