特发性膜性肾病肾组织中自噬相关蛋白LC3Ⅱ、P62和p-mTOR的表达及临床意义  

Expression and Clinical Significance of Autophagy Related Proteins LC3Ⅱ、P62 and p-mTOR in Idiopathic Membranous Nephropathy

作　　者：张翠 陈卫东 刘磊[1] 吴雪平[1] 潘艳[1] 常保超[1] ZHANG Cui;CHEN Weidong;LIU Lei(Department of Nephrology,the First Affiliated Hospital of Bengbu Medical College,Bengbu,233000)

机构地区：[1]蚌埠医学院第一附属医院肾病科,蚌埠233004

出　　处：《中国中西医结合肾病杂志》2023年第2期117-120,I0002,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：安徽省重点研究与开发计划项目(No.202004j07020011)。

摘　　要：目的:观察特发性膜性肾病(idiopathic membranous nephropathy,IMN)患者肾组织中自噬相关蛋白LC3Ⅱ、P62和p-mTOR的表达,了解IMN患者肾组织细胞自噬的溶酶体降解过程是否参与了IMN的发生发展。方法:选取蚌埠医学院第一附属医院2020年11月—2021年06月经肾活检初次确诊的IMN患者42例,免疫组化检测LC3Ⅱ、P62和p-mTOR的表达,分析两组间自噬蛋白表达差异性及IMN组自噬蛋白表达与患者肾间质纤维化及肾功能等临床相关指标的相关性。结果:与正常肾组织相比,IMN组的肾组织自噬相关蛋白LC3Ⅱ、P62和p-mTOR的表达显著增加(均为P<0.01);在IMN组中,与无纤维化组相比,有纤维化组LC3Ⅱ、P62的表达显著增加(均为P<0.05);IMN患者胆固醇(TC)、三酰甘油(TG)、24 h尿蛋白定量与肾组织LC3Ⅱ、P62和p-mTOR的表达成正相关。结论:IMN患者的肾组织中自噬体数量增加,自噬溶酶体数量不增加,自噬体的降解受阻,在IMN患者中,细胞自噬的溶酶体降解阶段受损所导致的自噬通量不足参与了IMN的发生发展。Objective:To observe the expression of autophagy-related proteins LC3Ⅱ、P62 and p-mTOR in the renal tissues of patients with idiopathic membranous Nephropathy(IMN),and to investigate whether the lysosomal degradation of autophagy is involved in the occurrence and development of IMN.Methods:42 IMN patients who were first diagnosed by renal biopsy from November 2020 to June 2021 in the First Affiliated Hospital of Bengbu Medical College were selected.The expressions of LC3Ⅱ、P62 and p-mTOR were detected by immunohistochemistry.The difference of autophagic protein expression between the two groups and the correlation between the expression of autophagic protein in IMN group and clinical indicators such as renal interstitial fibrosis and renal function were analyzed.Results:Compared with normal kidney tissue,the expression of autophagy related proteins LC3Ⅱ、P62 and p-mTOR were significantly increased in IMN group(P<0.01).In IMN group,compared with the non-fibrosis group,the expression of LC3Ⅱand P62 in the fibrosis group was significantly increased(P<0.05).The levels of cholesterol(TC),triglyceride(TG)and 24 h urinary protein in IMN patients were positively correlated with the expressions of LC3Ⅱ、P62 and p-mTOR in kidney tissue.Conclusion:The number of autophagosomes in renal tissues of IMN patients is increased,but the number of autophagolysosomes is not increased,and the degradation of autophagosome is blocked.In IMN patients,the insufficient autophagy flux caused by the impaired lysosome degradation stage of autophagy is involved in the occurrence and development of IMN.

关 键 词：特发性膜性肾病 溶酶体 自噬 肾间质纤维化 

分 类 号：R692[医药卫生—泌尿科学]