胃复春胶囊通过STAT3信号通路促进人胃癌细胞凋亡  

Apoptotic Effect of Weifuchun Capsule on Human Gastric Cancer Cells via STAT3 Pathway

作　　者：潘海春 祁大庆 陈琳慧 汪玲羽[2] 万林春 PAN Haichun;QI Daqing;CHEN Linhui(Hangzhou Huqingyutang Pharmaceutical Co.,Ltd,Hangzhou(311100),China)

机构地区：[1]杭州胡庆余堂药业有限公司,杭州311100 [2]浙江中医药大学附属第三医院 [3]江西省药品检验检测研究院

出　　处：《浙江中医药大学学报》2023年第3期227-233,共7页Journal of Zhejiang Chinese Medical University

基　　金：浙江省科技计划项目(2022CO3131)。

摘　　要：[目的]研究胃复春胶囊对人胃癌MKN-49P细胞的作用,为胃复春胶囊在抗消化道肿瘤方面的应用提供新的依据。[方法]受试样品为经胃复春胶囊饲喂大鼠的含药血清。胃癌MKN-49P细胞与不同浓度的胃复春胶囊含药血清共同孵育,采用噻唑蓝(methy thiazolyl tetrazolium,MTT)法及流式细胞术分别检测细胞活力和细胞凋亡情况;JC-1染色检测线粒体膜电位的变化;免疫印迹法检测信号传导与转录激活因子3(signal transducer and activator of transcription 3,STAT 3)蛋白及与细胞凋亡相关的蛋白表达情况。[结果]胃复春胶囊含药血清能够剂量和时间依赖地抑制MKN-49P细胞的生长,并可诱导MKN-49P细胞凋亡。浓度为0~100μg·mL-1的胃复春胶囊含药血清作用于MKN-49P细胞24 h后,细胞活力呈剂量依赖性降低;JC-1染色显示线粒体膜电位降低;经过25、50、100μg·mL-1的胃复春胶囊含药血清分别处理24 h后,胃复春胶囊含药血清处理组细胞中STAT3的磷酸化减弱。[结论]胃复春胶囊含药血清能抑制胃癌细胞的增殖并诱导其凋亡,其作用机制可能与STAT3信号通路有关,该结果为胃复春胶囊在临床上治疗胃癌提供了依据。[Objective]To study the effect of Weifuchun Capsule on human gastric cancer MKN-49P cells,and provide a new basis for the application of Weifuchun Capsule in anti-gastrointestinal cancer.[Methods]The test sample was the serum of rats fed with Weifuchun Capsule.Gastric cancer MKN-49P cells were incubated with different concentrations of drug-containing serum.Cell viability and apoptosis were detected by methy thiazolyl tetrazolium(MTT)assay and flow cytometry separated.JC-1 staining was applied to detect the changes of mitochondrial membrane potential(MMP).The expression levels of signal transducer and activator of transcription 3(STAT3)and apoptosis related proteins were detected by Western blot.[Results]Weifuchun Capsule containing serum could inhibit the growth of MKN-49P cells in a dose and time-dependent manner,and induce MKN-49P cells apoptosis.The cell viability of MKN-49P cells decreased in a dose-dependent manner after treated with 0~100μg·mL-1 Weifuchun Capsule containing serum for 24 h.The MMP and phosphorylation of STAT3 were attenuated after treated with Weifuchun Capsule contained serum at the concentration of 25,50,100μg·mL-1 for 24 h.[Conclusion]Weifuchun Capsule can inhibit the proliferation and induce apoptosis of gastric cancer cells,and its mechanism may be related to the STAT3 pathway.The present work provides a basis for the clinical treatment of gastric cancer with Weifuchun Capsule.

关 键 词：胃复春胶囊 含药血清 胃癌 MKN-49P细胞 STAT3信号通路 凋亡 剂量依赖 时间依赖 

分 类 号：R256[医药卫生—中医内科学]