APOBEC3G对新型布尼亚病毒复制能力的影响  

Effects of APOBEC3G on the replications of severe fever with thrombocytopenia syndrome virus

作　　者：陶琰心 黎安玲[1] 冉若曦 段勇威 杨桂[1] 刘松梅[1] TAO Yanixn;LI Anling;RAN Ruoxi;DUAN Yonywei;YANG Gui;LIU Songmei(Clinical Laboratory of Wuhan University Zhongnan Hospital/Gene Diagnostic Center,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院检验科/基因诊断中心,湖北武汉430071

出　　处：《中国输血杂志》2023年第4期285-289,共5页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金资助项目(81972009,82172359)。

摘　　要：目的研究载脂蛋白B mRNA编辑酶催化亚单位3G(APOBEC3G)对新型布尼亚病毒(severe fever with thrombocytopenia syndrome virus,SFTSV)复制及IL-6生成的影响。方法首先通过慢病毒感染方式构建稳定过表达APOBEC3G的A549细胞,再使用人源SFTSV感染APOBEC3G过表达A549细胞;RT-qPCR检测细胞内的APOBEC3G、SFTSV-非结构蛋白编码基因(non-structural protein,NS)、白介素-6(IL-6)的mRNA表达量;流式荧光法检测细胞培养液上清中IL-6蛋白水平。结果SFTSV感染A549细胞可诱导APOBEC3G、IL-6 mRNA分别显著上调约100、800倍(P均<0.001);过表达APOBEC3G的A549细胞感染SFTSV后,细胞内SFTSV的NS mRNA表达下调57%(P=0.001)、IL-6 mRNA表达上调约58%(P=0.001),培养液上清中IL-6蛋白水平升高[对照组为(11257.92±274.36)pg/mL,过表达APOBEC3G组为(15150.65±215.54)pg/mL,P<0.001]。结论SFTSV感染细胞后APOBEC3G表达水平升高,过表达APOBEC3G可抑制SFTSV复制,并可促进IL-6的生成。Objective To study the effect of apolipoprotein B mRNA editing enzyme catalytic subunit 3G(APOBEC3G)on virus replication and IL-6 production during severe fever with thrombocytopenia syndrome virus(SFTSV)infection.Methods Lentiviral infection was used to establish A549 cell lines that overexpress APOBEC3G,which were then infected with patient-derived SFTSV.RT-qPCR was used to detect the mRNA expression of APOBEC3G,non-structural protein-encoding gene of SFTSV(non-structural protein,NS)and IL-6.Moreover,protein level of IL-6 in the culture supernatant was determined by immunofluorescence and flow cytometry.Results SFTSV infection significantly induced mRNA expression of APOBEC3G and IL-6 by about 100 and 800 times respectively(P<0.001).The mRNA expression of NS gene was down-regulated by 57% after APOBEC3G overexpression(P=0.001),while IL-6 mRNA expression was up-regulated by 58%(P=0.001).Protein level of IL-6 in the supernatant of APOBEC3G-overexpressing cells(15150.65±215.54)was increased in comparison to the control[(11257.92±274.36)pg/mL,P<0.001)].Conclusion SFTSV infection could induce APOBEC3G expression that,in turn,inhibit the replication of SFTSV and promote the production of IL-6.

关 键 词：新型布尼亚病毒 载脂蛋白B mRNA编辑酶催化亚单位3G 白介素6 

分 类 号：R373[医药卫生—病原生物学]