应用双泵联动自动化设备进行急性等容稀释自体血采集对实验动物血流动力学指标的影响  

Acute normovolemic hemodilution autologous blood collection with two pumps linked automatic equipment and its influence on hemodynamic parameters in laboratory animals

作　　者：郝兴海[1] 路阳[2] 韩爱民 金振华 贾明[1] HAO Xinghai;LU Yang;HAN Aimin;JIN Zhenhua;JIA Ming(Department of Cardiac Surgery,Beijing Anzhen Hospital,Capital Medical University,Beijing 100029,China;Department of Stomatology,Peking University Third Hospital;Shandong Academy of Pharmaceutical Sciences)

机构地区：[1]首都医科大学附属北京安贞医院心脏外科,北京100029 [2]北京大学第三医院口腔科 [3]山东省药学科学院

出　　处：《中国输血杂志》2023年第4期306-309,共4页Chinese Journal of Blood Transfusion

基　　金：北京市属医院科研培育项目(PX2016004)。

摘　　要：目的探讨应用双泵联动自动化设备进行急性等容稀释(acute normovolemic hemodilution,ANH)自体血采集在提高采血速度、精确控制等容比例和稳定实验动物血流动力学方面的作用及可行性。方法建立16头巴马小型猪ANH自体血采集动物实验模型,监测记录心率和血压指标,每个模型分别应用人工方式和自动化设备方式进行ANH操作。对比2种方式的采血速度、稀释液输注总量和等容稀释精确度。观察操作前后实验动物的血流动力学参数变化情况。结果人工组采血速度(10.0~26.7)g/min,平均(16.0±5.6)g/min,输注稀释液量(260~550)g,平均(385.4±76.2)g;设备组采血速度平均(40.4±0.6)g/min,用时(10~10.2)min,平均(10.1±0.0)min,输注稀释液量(392~414)g,平均(405.9±6.1)g。设备组采血速度较人工组快,完成时间用时短(P<0.05)。人工组操作前后心率平均值由(111.6±9.6)次/min加快至(116.0±10.6)次/min(P<0.05),收缩压平均值由(98.9±8.3)mmHg变化至(92.9±8.4)mmHg(P<0.05),但是平均压和舒张压无差异(P>0.05)。设备组操作前后相关血流动力学参数均无差异(P>0.05)。结论应用双泵联动自动化设备进行ANH自体血采集安全、可行,在采血速度、控制等容比例和稳定血流动力学参数方面较人工方式有一定优势。Objective To explore the effect and feasibility of using two pumps linked automatic equipment to collect autologous blood of acute normovolemic hemodilution(ANH)in improving blood collection speed,accurately controlling the proportion of normovolemic hemodilution and stabilizing the hemodynamics of laboratory animals.Methods Sixteen Bama miniature pigs were monitored and recorded of heart rate and blood pressure as laboratory animal model underwent ANH autologous blood collection with both the methods of manual mode and automatic equipment mode.The blood collection speed,total amount of hemodilution solution and accuracy of normovolemic proportion control of the two methods were compared.The changes of hemodynamic parameters before and after ANH blood collection were observed and compared between the two methods.Results In the manual group,the blood collection speed was(10-26.7)g/min,with an average of(16.0±5.6)g/min,and the amount of hemodilution solution was(260-550)g,with an average of(385.4±76.2)g;in the automatic equipment group,the average blood collection speed was(40.4±0.6)g/min,which was significantly faster(P<0.05),and the collection time was(10-10.2)min,with an average of(10.1±0.0)min,which was significantly shorter(P<0.05),and the amount of hemodilution solution was(392-414)g,with an average of(405.9±6.1)g.There were significant changes in heart rate,from(111.6±9.6)bpm to(116.0±10.6)bpm and systolic artery pressure(SAP)from(98.9±8.3)mmHg to(92.9±8.4)mmHg before and after the manual ANH blood collection,but mean artery pressure(MAP)and diastolic artery pressure(DAP)did not change significantly.There were no significant changes in hemodynamic parameters in the automatic equipment group before and after the autologous blood collection.Conclusion It is safe and feasible for ANH with two pumps linked automatic equipment,which has advantages over manual method in terms of blood collection speed,control of normovolemic hemodilution proportion and stability of hemodynamic parameters.

关 键 词：急性等容稀释 自体血 自动化设备 血流动力学 动物实验 

分 类 号：R457.1[医药卫生—治疗学] R331.3[医药卫生—临床医学]