单细胞转录组测序技术及其在动物科学领域的应用  

Single-cell Transcriptome Sequencing Technology and Its Application in the Field of Animal Science

作　　者：娄姬英 郭其新[1] 毕瑜林[1] 江勇 王志秀 陈国宏[1,2] 白皓 常国斌 LOU Ji-Ying;GUO Qi-Xin;BI Yu-Lin;JIANG Yong;WANG Zhi-Xiu;CHEN Guo-Hong;BAI Hao;CHANG Guo-Bin(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;Joint International Research Laboratory of Agriculture and Agri-Product Safety,The Ministry of Education,Institutes of Agricultural Science and Technology Development,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]扬州大学农业科技发展研究院教育部农业与农产品安全国际合作联合实验室,扬州225009

出　　处：《农业生物技术学报》2023年第5期1074-1087,共14页Journal of Agricultural Biotechnology

基　　金：现代农业产业技术体系建设专项资助(CARS-41);江苏省研究生科研创新计划(KYXCX21_3258)。

摘　　要：单细胞测序是一个新兴的研究领域,主要包括转录组测序、全基因组测序以及表观基因组测序。单细胞转录组测序(single-cell RNA-sequencing,scRNA-seq)是指在单个细胞水平上对RNA进行高通量测序和分析的一项新技术。近年来,单细胞相关实验技术发展迅速,目前已被广泛应用于生物及医学领域,主要用于研究生物进化过程、组织器官发育、肿瘤疾病发生和精准医疗诊断等,然而在动物科学领域的研究及其应用相对较少,目前大多停留在对动物生理和进化发育层面的研究。本文就单细胞转录组测序技术方法及其在动物科学领域的引用进行综述,以期为单细胞转录组测序技术的发展及其在动物科学领域的应用提供理论依据。Single-cell sequencing is a new research area,which mainly includes transcriptome sequencing,whole genome sequencing,and epigenomic sequencing.Single-cell RNA-sequencing(scRNA-seq)refers to a new technique for high-throughput sequencing and analysis of RNA at the single cell level.Recently,singlecell related experimental technology has developed rapidly and has been widely used in the field of biology and medicine,mainly for the study of biological evolution process,tissue and organ development,tumor disease occurrence and precision medical diagnosis.However,few studies were reported in the field of animal science research and its application,currently,most of them focus on the field of animal physiology and evolutionary development.This article summarized the technical methods of single-cell transcriptome sequencing and its application in the field of animal science.This review provides a theoretical basis for the development of single-cell transcriptome sequencing technology and its application in the field of animal science.

关 键 词：单细胞转录组测序(scRNA-seq) 细胞分离 单细胞悬液 动物科学 

分 类 号：S811.6[农业科学—畜牧学]