川穹嗪通过减弱血脑屏障损伤改善老年小鼠术后神经认知障碍  

Tetramethylpyrazine improves postoperative neurocognitive disorders in old mice by attenuating blood-brain barrier injury

作　　者：李光耀 李江静 魏顺民 井紫薇 吕苗苗 张霞婧 高昌俊 孙绪德 LI Guangyao;LI Jiangjing;WEI Shunmin;JING Ziwei;LYU Miaomiao;ZHANG Xiajing;GAO Changjun;SUN Xude(Department of Anesthesiology,Tangdu Hospital,Air Force Medical University,Xi'an 710038,China;Department of Anesthesiology,Xijing 986 Hospital,Air Force Medical University,Xi'an 710054,China)

机构地区：[1]空军军医大学唐都医院麻醉科,陕西西安710038 [2]空军第九八六医院麻醉科,陕西西安710054

出　　处：《空军军医大学学报》2023年第4期301-304,共4页Journal of Air Force Medical University

基　　金：国家自然科学基金(31570845);陕西省重点研发计划项目(2022SF-189);陕西省自然科学基金(S2019-JC-YB-0553)。

摘　　要：目的 探讨川穹嗪(TMP)对老年小鼠脾切除术后神经认知障碍的影响及机制。方法 将老年小鼠随机分为对照+生理盐水组(Sham组)、模型+生理盐水组(Con组)、模型+川穹嗪组(TMP组),TMP组腹腔注射TMP(40 mg/kg)7 d, Sham组和Con组腹腔注射等剂量的生理盐水,通过脾切除方法建立小鼠神经认知障碍模型。通过新物体识别实验检测小鼠记忆能力,伊文思蓝(EB)实验检测小鼠血脑屏障(BBB)通透性,电镜观察小鼠BBB微观改变,苏木精-伊红(HE)染色观察小鼠海马CA1区神经元改变。结果 与Sham组相比,Con组小鼠识别指数(DI)明显降低(P<0.05),脑内EB含量明显上升(P<0.05),电镜下BBB破坏明显,HE染色海马CA1区神经元炎症反应明显;与Con组相比,TMP组小鼠DI值明显上升(P<0.05),脑内EB含量明显降低(P<0.05),BBB破坏减轻,海马CA1区神经元炎症反应减轻。结论 TMP通过减弱BBB损害改善老年小鼠脾切除术后神经认知障碍。Objective To investigate the effect and mechanism of tetramethylpyrazine(TMP)on postoperative neurocognitive disorders after splenectomy in old mice.Methods Old mice were randomly divided into control+normal saline group(Sham group),model+normal saline group(Con group)and model+tetramethylpyrazine group(TMP group).TMP group was intraperitoneally injected with TMP(40 mg/kg)for 7 d,and Sham group and Con group were intraperitoneally injected with equal dose of normal saline.The mouse model of neurocognitive disorders was established by splenectomy.The memory ability of mice was detected by novel object recognition test.The blood-brain barrier(BBB)permeability of mice was detected by Evans blue(EB)assay,the microscopic changes of BBB were observed by electron microscope,and the changes of neurons in hippocampal CA1 region were observed by hematoxylin-eosin(HE)staining.Results Compared with Sham group,the discrimination index(DI)of mice in Con group was significantly decreased(P<0.05),EB content in brain was significantly increased(P<0.05),the BBB was significantly damaged under electron microscope,and HE staining showed obvious neuronal inflammation in hippocampal CA1 region.Compared with Con group,DI in TMP group was significantly increased(P<0.05),EB content in brain was significantly decreased(P<0.05),the BBB disruption was attenuated,and the neuronal inflammation in hippocampal CA1 region was alleviated.Conclusion TMP improves postoperative neurocognitive disorders in old mice after splenectomy by attenuating BBB disruption.

关 键 词：川穹嗪 神经认知障碍 血脑屏障 

分 类 号：R285.5[医药卫生—中药学]