柴胡龙骨牡蛎汤对心肌梗死伴焦虑大鼠肠道屏障及LPS/NF-κB介导的心肌炎性损伤的影响  

Effects of Chaihu Longgu Muli Decoction on intestinal barrier and LPS/NF-κB mediated myocardial inflammatory injury in rats with myocardial infarction and anxiety

作　　者：王昀 史金玉 赵海滨[3] 王晨 王超[3] 侯季秋 刘鹏 邱新萍 WANG Yun;SHI Jinyu;ZHAO Haibin;WANG Chen;WANG Chao;HOU Jiqiu;LIU Peng;QIU Xinping(Shunyi Hospital,Beijing Hospital of Traditional Chinese Medicine,Beijing 101300,China)

机构地区：[1]北京中医医院顺义医院科研处,101300 [2]北京中医药大学 [3]北京中医药大学东方医院心内科

出　　处：《环球中医药》2023年第4期661-667,共7页Global Traditional Chinese Medicine

基　　金：北京市自然科学基金(7214298)。

摘　　要：目的观察柴胡加龙骨牡蛎汤(CHLM)对心肌梗死合并焦虑大鼠肠道屏障、内毒素(lipopolysaccharides,LPS)/核因子κB(nuclear transcription factor-κB,NF-κB)介导的心肌炎性损伤、心脏收缩功能、行为学、血清炎症因子的影响。方法将24只雄性SD大鼠随机分为假手术组、模型组、中药组。其中模型组,中药组接受为期3周的焦虑造模,在第2周末模型组及中药组进行心梗造模。假手术组正常饮水,仅挂线,不结扎。术后第二天,中药组予柴胡加龙骨牡蛎汤13.6 g/kg灌胃。模型组及假手术组予等体积生理盐水灌胃。检测大鼠垂直运动分数、水平运动分数、开放臂进入次数百分比(the percentage of open arm entry,OE%)、开放臂进入时间百分比(the percentage of open arm time,OT%)等行为学指标,检测大鼠左室射血分数(left ventricular injection fraction,LVEF)、左室缩短分数(left ventricular systolic function,LVFS)、左心室舒张末期内径(left ventricular internal diameter at end-diastole,LVIDd)和左心室收缩末期内径(left ventricular internal diameter at end-systole,LVIDs)等超声指标。对心脏组织石蜡切片进行苏木精—伊红(hematoxylin-eosin,HE)染色后观察。用酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)测定血清LPS及炎性因子水平,用蛋白免疫印迹法(Western-blot,WB)检测心肌组织NF-κB信号通路相关蛋白表达。结果(1)取样前,假手术组7只大鼠存活,模型组,中药组各6只大鼠存活。(2)肠道屏障指标:与模型组相比,中药组闭合蛋白(occludin)、闭锁连接蛋白-1(zonula occludens-1,ZO-1)表达升高,差异具有显著统计学意义。中药组紧密连接蛋白-1(claudin-1)表达相对模型组有升高趋势,但差异无统计学意义。(3)NF-κB相关蛋白:与模型组相比,中药组NOD样受体热蛋白结构域相关蛋白3(NOD-like receptor thermal protein domain associated protein 3,NLRP3)、磷酸化的核因子κB p65与核因子κB p65Objective To observe the effects of Chaihu Longgu muli decoction(CHLM)on intestinal barrier,LPS/NF-κB mediated myocardial Inflammatory Injury,cardiac function,behavior,serum endotoxin(LPS)and inflammatory factors in rats with myocardial infarction and anxiety.Methods 24 male SD rats were randomly divided into sham operation group,model group and CHLM group.The model group and CHLM group received empty bottle stimulation anxiety modeling for 3 weeks.On the last day of the second week,the model group and the CHLM group received myocardial infarction modeling.The sham operation group drank water normally,and only hung the line on the 14th day without ligation.On the second day after operation,the CHLM group was given CHLM by gavage of 13.6g/kg.The model group and the sham operation group were perfused with equal volume of normal saline.The levels of vertical movement score,horizontal movement score,proportion of open arm entry(OE%)/proportion of open arm time(OT%)were tested to assess anxiety.Left Ventricular Internal Diameter in Diastole(LVIDd),Left Ventricular Internal Dimension systole(LVIDs),left ventricular ejection fraction(LVEF)and left ventricular shortening fraction(LVFS)were measured by echocardiography.Myocardial tissue pathological staining and immunohistochemistry(IHC)was used to objectively show the inflammatory infiltration and collagen deposition of myocardial cells in each group.ELISA was used to determine serum LPS and inflammatory factors(IL-6,IL-1β、TNF-α)level.The expression of NFκB signal pathway related proteins in myocardial tissue were detected by Western blot and IHC.Results(1)Before sampling,7 rats in the sham operation group survived,and 6 rats in each of the other groups survived.(2)Intestinal barrier index:compared with the model group,the expression of occludin and ZO-1 in the traditional Chinese medicine group was increased,and the difference was statistically significant.Compared with the model group,the expression of Claudin-1 in the traditional Chinese medicine group was incre

关 键 词：柴胡加龙骨牡蛎汤 心肌梗死 焦虑 肠道屏障 炎症 

分 类 号：R285.5[医药卫生—中药学]