基于指纹图谱及多成分含量测定的泽泻汤单煎及共煎差异性研究  

Difference between single decoction and total decoction of Zexie decoction based on fingerprint and multicomponent content determination

作　　者：赵美奇 武慧敏 徐丽芝 孟扬 陈卫东 杨瑞 王淑君[5] ZHAO Mei-qi;WU Hui-min;XU Li-zhi;MENG Yang;CHEN Wei-dong;YANG Rui;WANG Shu-jun(School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012;Yangtze Delta Drug Advanced Research Institute,Nantong Jiangsu 226133;Jiangxi University of Chinese Medicine,Nanchang 330004;The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110034;School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016;Jiangsu Aidi Nano Biopharmaceutical Co.,Ltd.,Nantong Jiangsu 226100)

机构地区：[1]安徽中医药大学药学院,合肥230012 [2]南通市海门长三角药物高等研究院,江苏南通226133 [3]江西中医药大学,南昌330004 [4]辽宁中医药大学附属第二医院,沈阳110034 [5]沈阳药科大学药学院,沈阳110016 [6]江苏艾迪纳米生物医药有限公司,江苏南通226100

出　　处：《中南药学》2023年第4期963-968,共6页Central South Pharmacy

基　　金：国家自然科学基金项目(No.81973494)。

摘　　要：目的基于指纹图谱及多成分含量测定探讨泽泻汤各药味单煎及共煎的差异。方法建立HPLC指纹图谱,对共有峰进行归属与指认,同时测定3种指标性成分含量,并结合特征峰峰面积,分析白术单煎、泽泻单煎及共煎时化学成分的变化。结果15批样品共有14个共有峰,其中12个峰归属于泽泻,2个峰归属于白术,共指认了3个色谱峰,分别为白术内酯Ⅲ、23-乙酰泽泻醇B、23-乙酰泽泻醇C;单煎、共煎23-乙酰泽泻醇B含量差异不大,单煎时23-乙酰泽泻醇C、白术内酯Ⅲ含量分别为共煎的1.5、3.0倍;共煎时仅有2个特征峰峰面积大于单煎。结论建立的指纹图谱和含量测定方法准确、稳定、可行,可用于泽泻汤的质量控制。泽泻汤各药味单煎、共煎前后无新物质生成,从总体上看各成分含量单煎优于共煎,可为该方配方颗粒的开发提供数据参考。Objective To determine the difference between single decoction and total decoction of each medicinal component of Zexie decoction based on fingerprint and multicomponent content determination.Methods HPLC fingerprint of Zexie decoction was established,and the common peaks were attributed and identified.The contents of 3 effective components were determined.According to the characteristic peak area,the changes of chemical compositions in the single decoction and total decoction were analyzed.Results There were 14 common peaks in the 15 batches of samples,12 of which were attributed to Alismatis Rhizoma and the other 2 to Atractylodis Macrocephalae Rhizoma.Three common peaks were identified:atractylodeⅢ,23-acetyl alisol B and 23-acetyl alisol C,respectively.The content of 23-acetyl alisol B in single decoction and total decoction showed no significant difference,while the content of 23-acetyl alisol C and atractylodeⅢin the single decoction was 1.5 and 3.0 times that of in the total decoction.In the total decoction,areas of two characteristic peak areas were larger than those of the single decoction.Conclusion The method is accurate,stable and reliable,which can be used for the quality control of Zexie decoction.No new substance is found before and after the single decoction and total decoction.The content of each component in the single decoction is better than that in the total decoction in general,which provides reference for the development of formula granules of this decoction.

关 键 词：泽泻汤 经典名方 单煎 共煎 指纹图谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]