解决夹心式侧流免疫层析中钩效应的问题:回顾及对未来解决方案的影响  

Addressing the problem of hook effect in sandwich lateral flow immunoassay: review and impact on future solutions

作　　者：刘尚 叶雅冰 何康松 唐建斌[1] Liu Shang;Ye Yabing;He Kangsong;Tang Jianbin(Zhejiang University,College of Chemical and Biological Engineering,Institute of Bioengineering,,Hangzhou 310058,China;Zhejiang University,Polytechnic Institute(Taizhou),Materials and Chemical engineering,,Hangzhou 310058,China)

机构地区：[1]浙江大学化学工程与生物工程学院生物工程研究所,杭州310058 [2]浙江大学工程师学院台州分院,杭州310058

出　　处：《中华检验医学杂志》2023年第4期415-420,共6页Chinese Journal of Laboratory Medicine

摘　　要：侧流免疫层析技术(LFIA)是一种快速检测技术,是将抗原抗体反应从试管或者实验器皿中移动到试纸条上进行,利用试纸条的层析作用使待测溶液向指定方向移动进而完成整个抗原抗体特异性反应,通过肉眼观察试剂条特定位置的颜色变化即可作出定性判断。因其具有快速、简便、特异性强、经济、无需专业人员的优点,现已广泛应用于医学检测、食品质量监测、环境监测、农业和畜牧业等领域。目前,阻碍LFIA技术发展的一大重要瓶颈就是钩效应的影响。本文旨在归纳总结目前应对钩效应的方法、手段及最新研究进展,以期为研究者在设计试纸条、对适宜纳米颗粒的选择及实现LFIA定量检测提供有力的技术参考。Lateral flow immunoassay(LFIA)is a rapid detection technique that allows researchers to move the antigen-antibody reaction from a test tube or laboratory vessel to a test strip.Due to the chromatographic effect of the test strip,the solution would move to a specified direction based on the test and complete the whole antigen-antibody specific reaction.A qualitative judgment can be made with the naked eye by observing the color change of the reagent strip at a specific location.Because of its advantages of being fast,simple,specific,inexpensive,and requiring no specialized personnel,LFIA is now widely used in medical testing,food quality monitoring,environmental monitoring,agriculture and animal husbandry.A major bottleneck for the development of LFIA technology is the hook effect.This paper summarizes the current methods,means and research progresses to combat the hook effect,hoping to provide a strong technical reference for researchers to design test strips,select suitable nanoparticles,and achieve quantitative LFIA detection.

关 键 词：免疫测定 纳米粒子 胶体金 假阴性反应 

分 类 号：Q503[生物学—生物化学]