A benzenesulfonic acid-modified organic polymer monolithic column with reversed-phase/hydrophilic bifunctional selectivity for capillary electrochromatography  

作　　者：Yikun Liu Ning He Yingfang Lu Weiqiang Li Xin He Zhentao Li Zilin Chen 

机构地区：[1]Department of Orthopedics Trauma and Microsurgery,Zhongnan Hospital of Wuhan University,School of Pharmaceutical Sciences,Wuhan University,Wuhan,430072,China [2]Key Laboratory of Combinatorial Biosynthesis and Drug Discovery,Ministry of Education,Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals,Wuhan,430071,China

出　　处：《Journal of Pharmaceutical Analysis》2023年第2期209-215,共7页药物分析学报（英文版）

基　　金：the National Natural Science Foundation of China(Grant Nos.:82273885,82073808 and 81872828).

摘　　要：Here,a styrene-based polymer monolithic column poly(VBS-co-TAT-co-AHM)with reversed-phase/hydrophilic interaction liquid chromatography(RPLC/HILIC)bifunctional separation mode was success-fully prepared for capillary electrochromatography by the in situ polymerization of sodium p-styrene sulfonate(VBS)with cross-linkers 3-(acryloyloxy)-2-hydroxypropyl methacrylate(AHM)and 1,3,5-triacryloylhexahydro-1,3,5-triazine(TAT).The preparation conditions of the monolith were optimized.The morphology and formation of the poly(VBS-co-TAT-co-AHM)monolith were confirmed by scanning electron microscopy(SEM)and Fourier transform infrared spectroscopy(FT-IR).The separation perfor-mances of the monolith were evaluated systematically.It should be noted that the incorporation of VBS functional monomer can provideπ-πinteractions,hydrophilic interactions,and ion-exchange in-teractions.Hence,the prepared poly(VBS-co-TAT-co-AHM)monolith can achieve efficient separation of thiourea compounds,benzene series,phenol compounds,aniline compounds and sulfonamides in RPLC or HILIC separation mode.The largest theoretical plate number for N,N0-dimethylthiourea reached 1.7×10^(5)plates/m.In addition,the poly(VBS-co-TAT-co-AHM)monolithic column showed excellent reproducibility and stability.This novel monolithic column has great application value and potential in capillary electrochromatography(CEC).

关 键 词：Sodium p-styrene sulfona Stationary phase Monolithic column Capillary electrochromatography Bifunctional interaction 

分 类 号：O657.7[理学—分析化学]