血流感染O139群霍乱弧菌的耐药性及基因组特征分析  

Drug resistance and genomic characteristics of a strain of O139 Vibrio cholerae isolated from human bloodstream infection

作　　者：李杨 逄波[2] 杜小莉[3] 胡锦瑞 李臻鹏 陈延莹 王玥 崔志刚[3] 周海健[3] 阚飙 Li Yang;Pang Bo;Du Xiaoli;Hu Jinrui;Li Zhenpeng;Chen Yanying;Wang Yue;Cui Zhigang;Zhou Haijian;Kan Biao(School of Public Health,Cheeloo College of Medicine,Shandong University,Jinan 250012,China;National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control,Beijing 102206,China;Laboratory Central Laboratory of Chinese Pathogen Identification Net,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]山东大学齐鲁医学院公共卫生学院,济南250012 [2]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京102206 [3]中国疾病预防控制中心传染病预防控制所国家致病菌识别网中心实验室,北京102206

出　　处：《中华预防医学杂志》2023年第4期542-549,共8页Chinese Journal of Preventive Medicine

基　　金：国家重点研发计划(2021YFC2302002)。

摘　　要：目的分析1株从菌血症患者血液中分离到的O139群霍乱弧菌产毒株的耐药性及基因组特征。方法使用肉汤稀释法和全自动药敏分析仪测定菌株的抗生素敏感性,使用二代基因测序和纳米孔测序获得菌株的完整基因组序列,使用BLAST在线比对与CARD、Resfinder、ISfinder、VFDB等数据库进行比对分析,明确菌株携带的耐药基因、插入序列和毒力基因等,使用MEGA 5.1软件构建基于核心基因组单核苷酸多态性的遗传系统发生树。结果霍乱弧菌SH400为O139群产霍乱毒素的菌株,携带了多个毒力相关基因和4个毒力岛。该菌株对链霉素、四环素、磺胺甲恶唑/甲氧苄啶耐药并携带了相应的耐药基因。该菌株携带了大小为172914 bp并且含有10种耐药基因的IncA/C型质粒。结合基因组进化关系发现,菌株间携带耐药基因和耐药质粒的情况呈现出一定聚集性。菌株SH400的传统ST型为ST69,cgMLST型为与cgST-252高度相似的新型别。结论该株O139群霍乱弧菌携带ctxAB毒力基因、多种耐药基因和IncA/C型质粒,且存在多重耐药岛。Objective To analyze the drug resistance and genomic characteristics of a strain of serogroup O139 Vibrio cholerae producing cholera toxin isolated from the bloodstream of a person with bacteremia.Methods The broth dilution method and automatic drug sensitivity analyzer were used to determine the antibiotic sensitivity of the strain.The complete genome sequence of the strain was obtained by using second-generation gene sequencing and nanopore sequencing.BLAST software was used for comparison and analysis with CARD,Resfinder,ISfinder,VFDB,and other databases.The drug-resistant genes,insertion sequences and virulence genes carried by the strain were identified.MEGA 5.1 software was used to construct a genetic phylogenetic tree based on the core genomic single nucleotide polymorphisms.Results V.cholerae SH400,as the toxigenic strain,carried multiple virulence-related genes and four virulence islands.The strain was resistant to streptomycin,tetracycline and trimethoprim-sulfamethoxazole,carrying corresponding drug-resistant genes.The strain also carried IncA/C plasmid with the size of 172914 bp and contained 10 drug-resistant genes.Combined with the genomic evolutionary relationship,this study found that the drug-resistant genes and drug-resistant plasmids carried among strains showed certain aggregation.The traditional ST type of strain SH400 was ST69,and the cgMLST type was a new type highly similar to cgST-252.Conclusion This strain of serogroup O139 V.cholerae carries the ctxAB gene,multiple drug-resistant genes and IncA/C plasmid,and there are multiple drug-resistant islands.

关 键 词：弧菌 霍乱 血流感染 耐药性 基因组 耐药质粒 

分 类 号：R446.5[医药卫生—诊断学]