基于DGKα-PA信号轴探讨扶正祛邪方对人肺癌H292细胞增殖、迁移的影响  

Effects of Fuzheng Quxie Prescription on Proliferation and Migration of Human Lung Cancer H292 Cells Based on DGKα-PA Axis

作　　者：张祎稀 骆莹滨 吴建春[1] 李雁[1] ZHANG Yixi;LUO Yingbin;WU Jianchun;LI Yan(Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China)

机构地区：[1]上海中医药大学附属市中医医院,上海200071

出　　处：《中国中医药信息杂志》2023年第5期77-82,共6页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81973795、82174183);上海市浦江人才项目(2020PJD057);上海申康医院发展中心临床三年行动计划(SHDC2020CR4052)。

摘　　要：目的探讨扶正祛邪方调节DGKα-PA信号轴对人肺癌H292细胞增殖、迁移的影响及可能机制。方法将H292细胞分为对照组和扶正祛邪方低、中、高浓度组,扶正祛邪方低、中、高浓度组分别予3、6、12 mg/mL扶正祛邪方干预48 h,对照组予等体积培养基常规培养。克隆形成实验观察H292细胞增殖情况,细胞划痕实验、Transwell实验观察细胞迁移和侵袭情况,ELISA检测细胞磷脂酸(PA)含量,Western blot检测细胞二酰甘油激酶α(DGKα)、蛋白激酶B(AKT)、p-AKT和雷帕霉素机械靶蛋白(mTOR)表达。结果与对照组比较,扶正祛邪方低、中、高浓度组细胞克隆形成数明显减少(P<0.05,P<0.01),细胞迁移率明显降低(P<0.01),侵袭细胞数明显减少(P<0.05,P<0.01),细胞PA含量明显减少(P<0.01),DGKα、p-AKT、mTOR蛋白表达明显降低(P<0.05,P<0.01),且以扶正祛邪方高浓度组抑制作用最明显(P<0.01)。结论扶正祛邪方可能通过DGKα-PA信号轴抑制人肺癌H292细胞增殖、迁移,其机制与抑制下游AKT/mTOR信号通路有关。Objective To investigate the effects and possible mechanism of Fuzheng Quxie Prescription on regulating DGKα-PA signaling axis on the proliferation and migration of human lung cancer H292 cells.Methods H292 cells were divided into control group and Fuzheng Quxie Prescription low-,medium-and high-dosage groups.The Fuzheng Quxie Prescription low-,medium-and high-dosage groups were treated with 3,6 and 12 mg/mL Fuzheng Quxie Prescription for 48 hours,and the control group was routinely cultured with an equal volume of medium.Proliferation of H292 cells were observed by colony formation assay;cell scratch test and Transwell assays were used to observe cell migration and invasion;ELISA was used to detect the content of phosphatidylic acid(PA)in H292 cells;the protein expression of diacylglycerol kinaseα(DGKα),protein kinase B(AKT),p-AKT and mechanistic target of rapamycin(mTOR)were detected by Western blot.Results Compared with the control group,the number of cell clones in the Fuzheng Quxie Prescription low-,medium-and high-dosage groups decreased significantly(P<0.05,P<0.01),the cell migration rate significantly decreased(P<0.01),the number of invasive cells significantly decreased(P<0.05,P<0.01),the content of PA in cells significantly reduced(P<0.01),the protein expressions of DGKα,p-AKT and mTOR significantly decreased(P<0.05,P<0.01).The Fuzheng Quxie Prescription high-dosage group had the most obvious inhibitory effect(P<0.01).Conclusion Fuzheng Quxie Prescription can inhibit the proliferation and migration of human lung cancer H292 cells through the DGKα-PA signaling axis,and its mechanism may be related to the inhibition of downstream AKT/mTOR signaling pathway.

关 键 词：扶正祛邪方 增殖 迁移 磷脂酸 二酰甘油激酶α DGKα-PA信号轴 人肺癌H292细胞 

分 类 号：R285.5[医药卫生—中药学]