隔药饼灸对动脉粥样硬化兔胸主动脉VEGF-A、VEGFR-2及DLL4表达的影响  

Effects of Herbal Cake-separated Moxibustion on the Expressions of VEGF-A,VEGFR-2,and DLL4 in Aorta of Atherosclerosis Rabbits

作　　者：葛君芸[1] 谭舒怀 李芊 刘红华[2] 彭涵 陈昕羽 易洪芬 刘迈兰[1] GE Junyun;TAN Shuhuai;LI Qian;LIU Honghua;PENG Han;CHEN Xinyu;YI Hongfen;LIU Mailan(College of Acupuncture&Moxibustion and Tuina,Hunan University of Chinese Medicine,Changsha 410208,China;Nursing College of Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学针灸推拿学院,湖南长沙410208 [2]湖南中医药大学护理学院,湖南长沙410208

出　　处：《中国中医药信息杂志》2023年第5期104-108,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(82074559);湖南省自然科学基金(2020JJ5414、2021JJ40401);湖南省教育厅优秀青年基金项目(19B435、19B428);长沙市杰出创新青年培养计划项目(kq1905036)。

摘　　要：目的观察隔药饼灸对动脉粥样硬化兔胸主动脉血管内皮生长因子(VEGF)-A、血管内皮生长因子受体(VEGFR)-2、DLL4表达的影响,探讨其促进受损血管内皮修复的机制。方法将45只兔按随机数字表法分为正常组、模型组、隔药饼灸组、直接灸组和药饼敷贴组,每组9只。正常组采用普通饲料喂养,其余各组采用高脂饲料喂养8周制备动脉粥样硬化兔模型,造模成功后,各治疗组于“巨阙”和双侧“天枢”“丰隆”,或双侧“心俞”“肝俞”“脾俞”予相应处理,30 min/次,每日1次,连续8周。HE染色观察胸主动脉血管壁形态,Western blot检测胸主动脉组织VEGF-A、VEGFR-2、DLL4蛋白表达,ELISA检测血清VEGF含量。结果与正常组比较,模型组兔胸主动脉壁结构受损明显,胸主动脉组织VEGF-A、VEGFR-2、DLL4蛋白表达明显升高(P<0.01),血清VEGF含量明显增加(P<0.01);与模型组比较,隔药饼灸组兔胸主动脉内皮损伤好转,直接灸组和药饼敷贴组兔胸主动脉内皮病理变化有所减轻,隔药饼灸组、直接灸组和药饼敷贴组兔胸主动脉组织VEGFR-2蛋白表达明显降低(P<0.05,P<0.01),直接灸组和药饼敷贴组兔胸主动脉组织DLL4蛋白表达明显降低(P<0.05,P<0.01),隔药饼灸组和药饼敷贴组兔血清VEGF含量明显减少(P<0.01)。结论隔药饼灸可能通过下调动脉粥样硬化兔胸主动脉VEGF-A、VEGFR-2、DLL4表达,降低血清VEGF含量,促进受损血管内皮修复,从而减轻动脉粥样硬化病变。Objective To observe the effects of herbal cake-separated moxibustion on the expressions of VEGF-A,VEGFR-2 and DLL4 in the aorta of atherosclerosis rabbits;To explore the mechanism of herbal cake-separated moxibustion in promoting vascular endothelial repair.Methods Totally 45 rabbits were divided into normal group,model group,herbal cake-separated moxibustion group,direct moxibustion group,and herbal cake application group according to random number table method,with 9 rabbits in each group.The rabbits in normal group were fed with normal diet,and the rabbits in other groups were fed with high fat diet for 8 weeks to establish atherosclerosis rabbit model.After successful modeling,each administration group was given corresponding treatment in“Juque”(RN14),bilateral“Tianshu”(ST25),“Fenglong”(ST40),or bilateral“Xinshu”(BL15),“Ganshu”(BL18)and“Pishu”(BL20),30 min/time,once a day,for 8 consecutive weeks.HE staining was used to observe the morphology of the thoracic aortic vessel wall,Western blot was used to detect VEGF-A,VEGFR-2,DLL4 protein expressions in thoracic aorta tissue,serum VEGF content was detected by ELISA.Results Compared with the normal group,the structure of the thoracic aorta wall in the model group was significantly damaged,the expressions of VEGF-A,VEGFR-2,DLL4 protein and the content of serum VEGF in the model group significantly increased(P<0.01).Compared with the model group,the thoracic aortic endothelial injury of the rabbits in the herbal cake-separated moxibustion group was improved,and the pathological changes of the thoracic aortic endothelial of the rabbits in direct moxibustion group and the herbal cake application group were alleviated,the expression of VEGFR-2 protein in thoracic aorta of rabbits in herbal cake-separated moxibustion group,direct moxibustion group and herbal cake application group significantly decreased(P<0.05,P<0.01),the expression of DLL4 protein in thoracic aorta of the rabbits in direct moxibustion group and the herbal cake application grou

关 键 词：动脉粥样硬化 隔药饼灸 血管内皮生长因子-A 血管内皮生长因子受体-2 DLL4 兔 

分 类 号：R245.8[医药卫生—针灸推拿学]