蒙药红花水提物的毒性观察  

Toxicity of Carthamus tinctorius L.Water Extract

作　　者：双荣 其日格尔 乌日汉[2] 白梅荣[2,3] 拉喜那木吉拉 韩晓静 SHUANG Rong;Qirigeer;Wurihan;BAI Meirong;Laxinamujila;HAN Xiaojing(Affiliated Hospital of Inner Mongolia Minzu University,Tongliao 028000,China;Inner Mongolia Minzu University,Tongliao 028000,China;Key Laboratory of Mongolian Medicine Research and Development Project,Tongliao 028000,China)

机构地区：[1]内蒙古民族大学附属医院,通辽028000 [2]内蒙古民族大学,通辽028000 [3]蒙医药研发工程教育部重点实验室,通辽028000

出　　处：《世界中医药》2023年第7期979-982,共4页World Chinese Medicine

基　　金：内蒙古自治区本科直属高校2019年“双一流”建设专项资金蒙药科研创新团队建设项目(190302);蒙药安全性评价创新团队项目(MY20190003);国家民委-教育部蒙医药研发重点实验室开放课题(MDK2019063)。

摘　　要：目的:血清动态观察蒙药红花水提物(HS)对肝、心、肾的毒性作用。方法:将蒙药红花水提物分为低、中、高剂量组及正常组,分别灌胃3个月HS,每天灌胃1次,每14 d尾部采血1次,动态观察血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)、三酰甘油酯(TG)、乳酸脱氢酶(LDH)、肌酸激酶(CK)、尿素(Urea)、肌酐(Cr)水平的变化;3个月后处死大鼠,取肝、心、肺、肾、脾、胃、肠、脑组织做苏木精-伊红染色(HE)病理观察。结果:HS低、中、高剂量组持续用药3个月后大鼠血清GPT、GOT、TG、LDH、CK、Urea、Cr水平和组织病理切片结果发现对肝、心、肾基本无损伤。结论:根据血清GPT、GOT、TG、CK、LDH、Gr、Urea水平和组织病理学观察发现,服用HS(0.4767~8.5806 g/kg)长达3个月对肝、心、肾基本无毒性。Objective:To observe the toxicity of the water extract of the Mongolian medicinal herb Carthamus tinctorius L.(HS)to the liver,heart,and kidney.Methods:Rats were intragastrically administrated with low-,medium-,and high-dose HS water extracts once a day for 3 months.Blood was collected from the tail once every 14 days.The serum levels of glutamic-pyruvic transaminase(GPT),glutamic-oxalacetic transaminase(GOT),triglycerides(TG),lactic dehydrogenase(LDH),creatine kinase(CK),urea,and creatinine(Cr)were determined.Three months later,the rats were sacrificed and the liver,heart,lung,kidney,spleen,stomach,intestine,and brain were collected.Hematoxylin-eosin(HE)staining was employed for the observation of pathological changes.Results:The serum levels of GPT,GOT,TG,LDH,CK,Urea,and Cr and the tissue sections showed that the three months of treatment with low-,medium-,and high-dose HS water extracts basically did not cause injuries in the liver,heart or kidney.Conclusion:The administration with HS water extract within 0.4767~8.5806 g/kg for three months has no toxic or side effect on the liver,heart,and kidney.

关 键 词：蒙药红花水提物 长期毒性 肝 心 肾 灌胃 用药剂量 组织病理 

分 类 号：R29[医药卫生—民族医学]