蒙药黑苏嘎-25促进神经元骨架蛋白表达提高神经递质含量改善阿尔茨海默病小鼠症状  

Mongolian medicine Heisuga-25 promotes the expression of neuroskeletal protein,increases the content of neurotransmitter and improves thesymptomssof Alzheimer's disease in mice

作　　者：杨玉秋[1] 高玉峰[1] 王澈力莫格 YANG Yuqiu;GAO Yufeng;WANG Chelimoge(Affiliated Hospital of Inner Mongolia University for Nationalities,Tongliao 028007,China;College of Clinical Medicine(Mongolian Medicine),Inner Mongolia University for Nationalities,Tongliao 028007,China)

机构地区：[1]内蒙古民族大学附属医院脑病一科,内蒙古通辽028007 [2]内蒙古民族大学临床医学(蒙医)学院,内蒙古通辽028007

出　　处：《细胞与分子免疫学杂志》2023年第2期153-158,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：内蒙古自然科学基金项目(2017MS(LH)0821)。

摘　　要：目的探讨蒙药黑苏嘎-25治疗阿尔茨海默(AD)小鼠的效果及机制。方法6月龄快速老化模型SAMP8小鼠分为模型组、[360 mg/(kg·d)]黑苏嘎-25处理组、[90 mg/(kg·d)]黑苏嘎-25处理组、多奈哌齐对照组[0.92 mg/(kg·d)],每组15只,另选择15只6月龄正常衰老SAMR1小鼠作为空白对照组。模型组和空白对照组小鼠常规喂养,生理盐水灌胃,其他给药组按照给药剂量灌胃,所有组灌胃均每天1次,共15 d。给药完成后第1天~第5天,各组取3只小鼠采用Morris水迷宫试验检测各组小鼠逃避潜伏期,穿越平台次数和停留时间。尼氏染色观察尼氏体数量,免疫组织化学染色法检测微管相关蛋白2(MAP-2)、低分子量神经丝蛋白(NF-L)表达和分布,Western blot法检测MAP-2、NF-L蛋白水平。ELISA检测小鼠脑组织(皮层、海马)中神经递质乙酰胆碱(ACh)、五羟色胺(5-HT)、去甲肾上腺素(NE)、多巴胺(DA)的含量。结果与空白对照组相比,模型组逃避潜伏期延长,穿越平台次数和停留时间减少,尼氏体减少,MAP2和NF-L蛋白表达减少;与模型组比较,黑苏嘎-25给药组逃避潜伏期缩短,穿越平台次数和停留时间增加,尼氏体数量增加,MAP-2和NF-L蛋白表达增加。[360 mg/(kg·d)]黑苏嘎-25处理对上述指标的影响更明显。与空白对照组比较,模型组海马及皮层中ACh、NE、DA及5-HT的含量明显减少,与模型组比较,低剂量组、高剂量组、多奈哌齐对照组ACh、NE、DA及5-HT含量显著增加。结论蒙药黑苏嘎-25治疗可改善AD模型小鼠学习记忆能力,保护小鼠神经功能,可能与促进神经元骨架蛋白表达并提高递质含量有关。Objective To investigate the effect and mechanism of Mongolian medicine Heisuga-25 on Alzheimer's disease(AD)mice.Methods Six month old SAMP8 mice were divided into model group,Heisuga-25[360 mg/(kg·d),90 mg/(kg·d)]treatment group,and donepezil control group[0.92 mg/(kg·d)],with 15 mice in each group.Another 156-month-old normal aging SAMR1 mice were selected as blank control group.The mice in the model group and blank control group were fed with normal saline,and the other groups were gavaged according to the dosage.All groups were gavaged once a day for 15 days.From Day 1 to Day 5 after administration,three mice in each group were taken and Morris water maze test was been used to detect the escape latency,times for crossing the platform and the residence time were detected.Nissl staining was used to observe the number of Nissl bodies.Western blot analysis and immunohistochemistry were used to detect the expression of microtubule associated protein 2(MAP-2)and low molecular weight neurofilament protein(NF-L).ELISA was used to detect the contents of acetylcholine(ACh),5-hydroxytryptamine(5-HT),norepinephrine(NE)and dopamine(DA)in cortex and hippocampus of mice.Results Compared with the blank control group,the escape latency was significantly prolonged,while the model group showed a decrease in the number of crossing the plaform,residence time,Nissl bodies,and the protein expression of MAP-2 and NF-L.Compared with the model group,Heisuga-25 administration group exhibited an increase in the number of crossing the platform and residence time,Nissl bodies,and the protein expression of MAP-2 and NF-L,but a shortened escape latency.The effect of high-dose groupHeisuga-25[360 mg/(kg·d)]on the above indexes was more obvious.Compared with the blank control group,the contents of ACh,NE,DA and 5-HT in hippocampus and cortex were decreased in the model group.Compared with the model group,the low-dose group,high-dose group and donepezil control group all observed an increase in the contents of ACh,NE,DA and 5-HT.Conclusio

关 键 词：黑苏嘎-25 阿尔茨海默病(AD) 神经递质 微管相关蛋白2(MAP-2) 低分子量神经丝蛋(NF-L) 

分 类 号：R285.5[医药卫生—中药学] R322.81[医药卫生—中医学] R392-33