生物信息学分析神经元钙蛋白1(CALN1)在胶质瘤中表达的临床意义及其与免疫细胞浸润的相关性  

Bioinformatic analysis of the clinical significance of calneuron 1(CALN1)in glioma and its correlation with immune cell infiltration

作　　者：张瀚 张津豪 王涛 ZHANG Han;ZHANG Jinhao;WANG Tao(Department of Medical Genetics and Developmental Biology,Basic Medical College,Air Force Medical University,Xi'an 710032;Academy of Laboratory,Jilin Medical University,Jilin 132013,China)

机构地区：[1]空军军医大学基础医学院医学遗传学与发育生物学教研室,陕西西安710032 [2]吉林医药学院检验学院,吉林吉林132013

出　　处：《细胞与分子免疫学杂志》2023年第3期205-212,共8页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(82073361);肿瘤生物学国家重点实验室自主课题(CBSKL2019ZZ20);陕西省重点研发计划(2020SF-201)。

摘　　要：目的 采用生物信息学方法分析神经元钙蛋白1(CALN1)在胶质瘤中表达的临床意义及其在肿瘤免疫细胞浸润中的作用。方法 利用仙桃学术在线分析癌症基因组图谱(TCGA)数据库中CALN1基因在胶质瘤中的表达情况,采用Kaplan-Meier生存分析法评价其预后价值,利用受试者工作特征(ROC)曲线评价其临床诊断效能,采用基因集富集分析(GSEA)探究CALN1在胶质瘤中的潜在作用机制,探讨CALN1 mRNA与胶质瘤免疫细胞浸润的关系。结果 胶质瘤中CALN1表达显著降低,且其表达水平与肿瘤分级负相关。与对照组比,CALN1在异柠檬酸脱氢酶突变型和染色体1p/19q共缺失胶质瘤中表达水平显著升高。CALN1低表达的胶质瘤患者预后较差,整体存活率、疾病相关存活率和无进展间隔期显著降低。ROC曲线分析显示CALN1表达水平具有较好的临床诊断价值。GSEA基因富集结果提示CALN1表达水平与有丝分裂和中性粒细胞脱颗粒负相关。免疫浸润分析显示CALN1低表达组中2型辅助T(Th2)细胞、巨噬细胞和中性粒细胞显著升高。结论 胶质瘤中CALN1表达水平与预后正相关,其表达异常降低可导致具有促肿瘤作用的免疫细胞浸润。CALN1可作为胶质瘤预后判断的潜在标志物和治疗靶点。Objective To analyze the clinical significance of calneuron 1(CALN1)expression in glioma and its role in tumor immune cell infiltration by bioinformatics.Methods The expression of CALN1 gene in glioma in the Cancer Genome Atlas(TCGA)database was analyzed by Xiantao Academic Online.Kaplan-Meier survival analysis was used to evaluate its prognostic value,and receiver operating characteristic(ROC)curve was employed to evaluate its clinical diagnostic efficiency.Gene Set Enrichment Analysis(GSEA)was adopted to identify the potential mechanism of CALN1 in glioma.The relationship between CALN1 mRNA and glioma immune cell infitration was discussed.Results The expression of CALN1 decreased significantly in glioma,and its expression level was negatively correlated with tumor grade.Compared with the control group,the expression level of CALN1 in isocitrate dehydrogenase mutant and 1p/19q co-deletion gliomas increased significantly.Glioma patients with low expression of CALN1 had poor prognosis and significantly reduced overall survival,disease specific survival and progression-free interval.ROC curve analysis showed that CALN1 expression level had good clinical diagnostic value.The results of GSEA gene enrichment suggested that the expression level of CALN1 was negatively correlated with mitosis and neutrophil degranulation.Immunoinfiltration analysis showed that T helper type 2(Th2)cells,macrophages and neutrophils were significantly increased in the group with low expression of CALN1.Conclusion The expression level of CALN1 in glioma is positively correlated with the prognosis.The abnormal decrease of CALN1 expression may lead to the invasion of tumor-promoting immune cells.CALN1 can be used as a potential prognostic marker and therapeutic target for glioma.

关 键 词：癌症基因组图谱(TCGA) 胶质瘤 神经元钙蛋白1(CALN1) 免疫细胞浸润 

分 类 号：S853.53[农业科学—临床兽医学] Q297[农业科学—兽医学] R392[农业科学—畜牧兽医]