滩涂围垦区农田土壤中无机磷解磷菌的分离鉴定及解磷特性  

Isolation,identification and characteristics of phosphorus solubilizing bacteria in agricultural soil of the coastal reclamation mudflat

作　　者：龙锡恩 张欣 万少昱 周彤彤 张蛟[2] LONG Xien;ZHANG Xin;WAN Shaoyu;ZHOU Tongtong;ZHANG Jiao(School of Geographical Science,Nantong University,Nantong 226007,China;Jiangsu Yanjiang Institute of Agricultural Sciences,Rugao 226541,China)

机构地区：[1]南通大学地理科学学院,江苏南通226007 [2]江苏沿江地区农业科学研究所,江苏如皋226541

出　　处：《南通大学学报（自然科学版）》2023年第1期67-76,共10页Journal of Nantong University(Natural Science Edition)　

基　　金：国家自然科学基金面上项目(41773079);江苏省农业科技自主创新资金项目(CX(20)3118)。

摘　　要：植物根际促生菌对植物有效利用土壤养分具有重要贡献,但关于滩涂农田土壤中促生菌解磷功能方面的研究较少。为此,从南通市和宁波市滩涂围垦区农田耕层土壤中分离出5株具有促生作用的细菌,并就其溶磷功能开展研究。经16S rRNA基因序列鉴定,所分离菌株分别为:Microbacterium flavescens(Mib.flavescens)、Pseudomonas chlororaphis(Ps.chlororaphis)、Pseudomonas jessenii(Ps.jessenii)、Massilia varians(M.varians)和Brevundimonas nasdae(Brv.nasdae)。在不同碳源、氮源、碳氮摩尔比(C/N)、pH和温度下测定细菌解磷能力,结果表明:与对照组相比,Mib.flavescens、Brv.nasdae在中性条件下解磷能力优越,解磷量保持在80.27~85.51 mg/L之间;较其他菌株而言,Ps.jessenii在4~37℃环境中的解磷能力显著,解磷量保持在13.59~81.94 mg/L之间。这3株菌株具有良好的微生物肥料开发应用潜力,为日后其菌肥开发奠定了基础。Plant growth-promoting rhizobacteria(PGPR)have an important contribution to the plants nutrients uptake from soil,but less research has mentioned that how function of PGPR in farmland soil of tidal flat.In this study,5 PGPR strains were isolated from topsoil of paddy and maize-wheat rotation field in reclamation areas of Nantong and Ningbo,respectively.The strains were identified by 16S rRNA gene sequence as follows:Massilia varians(M.varians),Brevundimonas nasdae(Brv.nasdae),Microbacterium flavescens(Mib.flavescens),Pseudomonas chlororaphis(Ps.chlororaphis)and Pseudomonas jessenii(Ps.jessenii).Five factors including carbon source,nitrogen source,C/N,pH and temperature were used to test the phosphorus solubilizing ability of the isolated strains.Results show that compared with the control group,Mib.flavescens and Brv.nasdae show the best phosphorus-solubilizing activity under neutral conditions(80.27-85.51 mg/L),more phosphorus(13.59-81.94 mg/L)is released by Ps.jessenii at a wide range of temperature than other strains.These three strains have good potential application in microbial fertilizer,and provide a fundamental information for the development of microbial fertilizer in the future.

关 键 词：解磷菌 解磷功能 滩涂围垦 农田土壤 

分 类 号：Q93[生物学—微生物学]