SDC2和TFPI2基因甲基化联合检测在结直肠癌早期筛查中的应用价值  

The value of combined SDC2 and TFPI2 gene methylation testing in the early screening of colorectal cancer

作　　者：储梦真 唐宜桂 张敏[1] 胡媛媛[1] 徐家丹 张洋[1] 杨玉萍 汪安勇[1] 王中新[1] Chu Mengzhen;Tang Yigui;Zhang Min;Hu Yuanyuan;Xu Jiadan;Zhang Yang;Yang Yuping;Wang Anyong;Wang Zhongxin(Dept of Clinical Laboratory,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院检验科,合肥230022

出　　处：《安徽医科大学学报》2023年第4期682-686,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校自然科学研究项目(编号:KJ2017A208)。

摘　　要：目的 探讨粪便样本中硫酸类肝素蛋白多糖基因(SDC2)甲基化和组织因子途径抑制物2基因(TFPI2)甲基化联合检测在结直肠癌早期筛查中的应用价值。方法 选取106例结直肠癌患者(结直肠癌组)、75例进展期腺瘤患者(进展期腺瘤组)、35例非进展期腺瘤患者(非进展期腺瘤组)为研究对象,并以同期153例其他消化系统干扰性疾病患者、182例结肠镜检查结果为阴性者作为对照组,采用甲基化荧光定量PCR(qMSP)法对所有受试者的粪便标本进行SDC2和TFPI2基因甲基化检测。以结肠镜检查及病理检查结果为金标准,评估SDC2和TFPI2基因甲基化联合检测对结直肠癌和腺瘤检测的灵敏度和特异度。结果 106例结直肠癌患者中,甲基化联合检测灵敏度为93.4%;75例进展期腺瘤患者中,甲基化联合检测灵敏度为62.7%;35例非进展期腺瘤患者中,甲基化联合检测灵敏度为34.3%;SDC2和TFPI2基因甲基化联合检测对结直肠癌和腺瘤筛查的特异度为94.6%。结论 SDC2和TFPI2基因甲基化联合检测对结直肠癌及其早期病变具有较高的灵敏度,同时保持较高的特异度。Objective To investigate the value of combined stool syndecan-2(SDC2)and tissue factor pathway inhibitor 2(TFPI2)gene methylation testing in the early screening of colorectal cancer.Methods 106 patients with colorectal cancer(colorectal cancer group),75 patients with advanced adenoma(advanced adenoma group)and 35 patients with non-advanced adenoma(non-advanced adenoma group)were selected as study subjects,and 153 patients with other gastrointestinal disorders and 182 patients with negative colonoscopy results during the same period were selected as the control group.The quantitative methylation-specific PCR(qMSP)method was used to detect SDC2 and TFPI2 gene methylation in the stool specimens of all subjects.The sensitivity and specificity of the combined SDC2 and TFPI2 gene methylation assay for the detection of colorectal cancer and adenoma were evaluated using colonoscopy and pathology results as the gold standard.Results Among 106 patients with colorectal cancer,the sensitivity of combined methylation test was 93.4%;among 75 patients with advanced adenoma,the sensitivity of combined methylation test was 62.7%;among 35 patients with non-advanced adenoma,the sensitivity of combined methylation test was 34.3%;the specificity of the combined SDC2 and TFPI2 gene methylation test for colorectal cancer and adenoma screening was 94.6%.Conclusion The combined SDC2 and TFPI2 gene methylation test has high sensitivity for colorectal cancer and its early lesions,and it also maintains high specificity.

关 键 词：结直肠癌 甲基化 SDC2基因 TFPI2基因 

分 类 号：R446.13[医药卫生—诊断学]