5株植物内生真菌生物转化柠檬烯和月桂烯产物分析  

Analysis of Biotransformation Products of Limonene and Myrcene by Five Plant Endophytic Fungi

作　　者：杨道茂[1] 王奇志[1] 裴真巧 YANG Daomao;WANG Qizhi;PEI Zhenqiao(College of Chemical Engineering,Huaqiao University,Xiamen 361021,China)

机构地区：[1]华侨大学化工学院,福建厦门361021

出　　处：《华侨大学学报（自然科学版）》2023年第3期374-380,共7页Journal of Huaqiao University(Natural Science)

基　　金：国家级大学生科创项目(202110385039);福建省农业重点自然科学基金资助项目(2014N0026);福建省泉州市校(院)地协同创新专项(重点)项目(2014Z101);福建省个人和团队科技员项目(2022年度);中医药公共卫生服务补助专项(财社[2018]43);华侨大学高层次人才科研启动资金资助项目(Z15X0004)。

摘　　要：为了筛选具有转化利用单萜类化合物能力的微生物菌株,以5株植物内生真菌为生物催化剂,(+)-柠檬烯、(-)-柠檬烯、月桂烯为生物转化底物,结合气相色谱-质谱联用技术考察生物转化产物.结果表明:5株植物内生真菌具有生物转化(+)-柠檬烯、(-)-柠檬烯、月桂烯的能力;当底物为(+)-柠檬烯时,主要产物均为柠檬烯-1,2-二醇,其中,菌株Diaporthe Nitschke PS10的产量最高,达到4.57 g·L-1;当底物为(-)-柠檬烯时,主要产物为柠檬烯-1,2-二醇,产量为0.81~2.04 g·L-1;当底物为月桂烯时,转化产物有环氧罗勒烯、氧化芳樟醇、柠檬烯-1,2-二醇等化合物;植物内生真菌是一种很有发展潜力的生物催化剂.In order to screen microbial strains with the ability to transform and utilize monoterpene compounds,five plant endophytic fungi were used as biocatalysts,(+)-limonene,(-)-limonene and myrcene were used as biotransformation substrates,and the biotransformation products were investigated by Gas Chromatography-Mass Spectrometry.The results show that five plant endophytic fungi possess the ability to biotransform(+)-limonene,(-)-limonene and myrcene.When the substrate is(+)-limonene,the main product is limonene-1,2-diol,and the highest yield is 4.57 g·L-1 when the strain is Diaporthe Nitschke PS10.When the substrate is(-)-limonene,the main product is also limonene-1,2-diol,and the yield is 0.81 to 2.04 g·L-1.When the substrate is myrcene,the conversion products include myroxyde,linalool oxide,limonene-1,2-diol and other compounds.It is indicated that plant endophytic fungi are potential biocatalysts.

关 键 词：生物转化 内生真菌 柠檬烯 月桂烯 柠檬烯-1 2-二醇 

分 类 号：Q53[生物学—生物化学]